一、基本信息
陈纯琪，博士，教授，博士生导师
电子邮件：ccckate0722(AT)hubu.edu.cn
研究方向：免疫学、微生物学、结构生物学与酶学
研究领域：结构生物学、酶应用与改造、酶的机理研究
酶是所有生命活动的发动机，酶的结构与催化机理研究对于了解生物基本运作、疾病发生原因、药物开发、以及医药产业与绿色生物工艺发展具有重大意义。陈纯琪教授通过结构生物学方法(X光晶体学与冷冻电镜)解析酶的三维结构与底物结合的复合体结构，阐释催化机理，并开展基于结构的理性设计，改造酶的特征与活性。工作内容主要涵盖三个体系：(1) 新型萜类合成酶；(2) 天然与人造合成塑料降解酶；(3) 霉菌毒素分解酶。目前已在Nature Reviews Chemistry, Nature Catalysis, Immunity, Nature Communications, Angew. Chem. Intl. Ed., JACS, ACS Catalysis等SCI期刊发表论文80余篇，共21篇获选为封面文章，申请国内外专利26个，已授权16个。2020年获“湖北省楚天学者特聘教授”、“湖北省百人计划特聘专家”、“湖北省创新群体”、“天津市自然科学二等奖”(第二完成人)等。
二、教育背景
2018.06至今 jxf手机网页版，教授
2014.01–2018.5 中国科学院天津工业生物技术研究所，访问学者，高级台湾青年访问学者
三、工作经历
2012.01–2013.12 中国科学院微生物研究所，副研究员，高级台湾青年访问学者
2008.02–2011.12 台湾中研院特聘博士后
2003.09–2008.01 台湾大学，微生物学研究所，博士
1999.09–2001.07 台湾大学，医事技术学研究所，硕士
1995.09–1999.07 台湾大学，医事技术学系，学士
四、科研项目
1. 国家重点研发计划，绿色生物制造专项，2021YFC2100302，医药与食品工业酶创制与催化，2021-07至2024-06，68.7万，在研，子课题负责人；
2. 国家自然科学基金，面上项目，31971205，新型倍半萜合成酶晶体结构与催化机理研究，2020-01至2023-12，58万，在研，主持；
3. 国家自然科学基金，青年基金，31200677，免疫球蛋白样转物3受体鉴定：功能性以及结构性研究，2013-01至2015-12，25万，已结题，主持。
五、发表论文（#共同一作，*通讯作者）
1. Zhang, X.,# Shen, P.,# Zhao, J.,# Chen, Y., Li, X., Huang, J.-W., Zhang, L., Li, Q., Gao, C., Xing, Q., Chen, C.-C., Guo, R.-T.,* Li, A.*. Rationally controlling selective steroid hydroxylation via scaffold sampling of a P450 Family. ACS Catalysis, 2023, 13, 1280–1289.
2. Chen, Q.,# Li, B.,# Zhang, L.,# Chen, X., Zhu, X., Chen, F., Shi, M., Chen, C.-C., Yang, Y., Guo, R.-T.,* Liu, W., Xu, J., Zheng, G.*. Engineered imine reductase for larotrectinib intermediate manufacture. ACS Catalysis, 2022, 12 (23), 14795-14803.
3. Dai, L.,# Li, H.,# Huang, J.-W.,# Hu, Y.,# He, M., Yang, Y., Min, J., Guo, R.-T.,* Chen, C.-C.*. Structure-based rational design of a short-chain dehydrogenase/reductase for improving activity toward mycotoxin patulin. International Journal of Biological Macromolecules, 2022-12-01, 222: 421-428.
4. Wu, L.,# An, J.,# Jing, X.,# Chen, C.-C., Dai, L., Liu, W.,* Guo, R.-T.,* Nie, Y.*. Molecular insights into the regioselectivity of the Fe(II)/2-ketoglutarate-dependent dioxygenase-catalyzed C-H hydroxylation of amino acids. ACS Catalysis, 2022-09-08, 12: 11586-11596.
5. Zhang, L.,# Zhang, X.,# Min, J.,# Liu, B., Huang, J.-W., Yang, Y., Liu, W., Dai, L., Yang, Y., Chen, C.-C.,* Guo, R.-T.*. Structural insights to a bi-functional isoprenyl dihposphate synthase that can catalyze head-to-tail and head-to-middle condensation. International Journal of Biological Macromolecules, 2022-08-01, 214: 492-499.
6. Li, H.,# Yang, Y.,# Hu, Y.,# Chen, C.-C., Huang, J.-W., Min, J., Dai, L.,* Guo, R.-T.*. Structural analysis and engineering of aldo-keto reductase from glyphosate-resistant Echinochloa colona. Journal of Hazardous Materials, 2022-08-15, 436: 129191.
7. Malwal, S. R.,# Shang, N.,# Liu, W., Li, X., Zhang, L., Chen, C.-C., Guo, R.-T.,* Oldfield, E.*. A structural and bioinformatics investigation of a fungal squanlene synthase and comparisons with other membrane proteins. ACS Omega, 2022-06-17, 7 (26): 22601-22612.
8. Wang, T.,# Yang, Y.,# He, M., Liu, M., Huang, J.-W., Min, J., Chen, C.-C., Liu, Y.,* Zhang, L.,* Guo, R.-T.*. Structural insights into the cyclization of unusual brasilane-type sesquiterpenes. International Journal of Biological Macromolecules, 2022-04-30, 209: 1784-1791.
9. Dai, L.,# Zhang, X.,# Hu, Y.,# Shen, J., Zhang, Q., Zhang, L., Min, J., Chen, C.-C., Liu, Y.,* Huang, J.-W.,* Guo, R.-T.*. Structural and functional insights into a nonheme iron- and α-ketoglutarate-dependent halogenase that catalyzes chlorination of nucleotide substrates. Applied and Environmental Microbiology, 2022-04-18, 88 (9): e0249721.
10. Zeng, W.,# Li, X.,# Yang, Y.,# Min, J.,# Huang, J.-W., Liu, W., Niu, D., Yang, X., Han, X., Zhang, L., Dai, L., Chen, C.-C.,* Guo, R.-T.*. Substrate-binding mode of a thermophilic PET hydrolase and engineering the enzyme to enhance the hydrolytic efficacy, ACS Catalysis, 2022-02-18, 12: 3033-3040.
11. Chen, C.-C.,# Dai, M.,# Zhang, L.,# Zhao, J., Zeng, W., Shi, M., Huang, J.-W., Liu, W., Guo, R.-T.*, Li, A.*. Molecular basis for a toluene monooxygenase to govern substrate selectivity, ACS Catalysis, 2022-02-13, 12: 2831-2839.
12. Mao, Z.,# Xiao, H.,# Shen, P.,# Yang, Y.,# Xue, J., Yang, Y., Shang, Y., Zhang, L., Li, X., Zhang, Y., Du, Y., Chen, C.-C., Guo, R.-T.*, Zhang, Y.*. KRAS(G12D) can be targeted by potent inhibitors via formation of salt bridge. Cell Discovery, 2022-01-25, 8 (1): 5.
13. Hu, Y.,# Li, H.,# Min, J.,# Yu, Y., Liu, W., Huang, J.-W., Zhang, L., Yang, Y., Dai, L.*, Chen, C.-C.,* Guo, R.-T.*. Crystal structure and biochemical analysis of the specialized deoxynivalenol–detoxifying glyoxalase SPG from Gossypium hirsutum. International Journal of Biological Macromolecules, 2022-01-18, 200: 388-396.
14. 陈纯琪, 韩旭, 刘卫东, 马立新, 刘珂*，郭瑞庭*. 基于结构改造来源于大阪伊德氏杆菌 201-F6 的PET水解酶，生物工程学报，2021-09-25, 37 (9): 3268-3275.
15. Dai, L.,# Chang, Z.,# Yang, J., Liu, W., Yang, Y., Chen, C.-C., Zhang, L., Huang, J.-W.,* Sun, Y.,* Guo, R.-T.*. Structural investigation of a thermostable 1,2- β -mannobiose phosphorylase from Thermoanaerobacter sp. X-514. Biochemical and Biophysical Research Communications, 2021-09-21, 579: 54-61.
16. Dai, L.,# Qu, Y.,# Hu, Y.,# Min, J., Yu, X., Chen, C.-C., Huang, J.-W.,* Guo, R.-T.*. Catalytically inactive lytic polysaccharide monooxygenase PcAA14A enhances the enzyme-mediated hydrolysis of polyethylene terephthalate, International Journal of Biological Macromolecules, 2021-09-07, 190: 456-462.
17. Dai, L.,# Qu, Y.,# Huang, J.-W., Hu, Y., Hu, H., Li, S., Chen, C.-C.,* Guo, R.-T.*. Enhancing PET hydrolytic enzyme activity by fusion of the cellulose-binding domain of cellobiohydrolase I from Trichoderma reesei. Journal of Biotechnology, 2021-05-24, 334: 47-50.
18. Liu, W.,# Ma, C.,# Liu, W.,# Zheng, Y., Chen, C.-C., Liang, A., Luo, X., Li, Z., Ma, W., Song, Y.,* Guo, R.-T.,* Zhang, T.*. Functional and structural investigation of a novel β-mannanase BaMan113A from Bacillus sp. N16-5. International Journal of Biological Macromolecules, 2021-04-15, 182: 899-909.
19. Chen, C.-C.,# Han, X.,# Li, X.,# Jiang, P., Niu, D., Ma, L., Liu, W., Li, S., Qu, Y., Hu, H., Min, J., Yang, Y., Zhang, L., Zeng, W., Huang, J.-W.,* Dai, L.,* Guo, R.-T.*. General features to enhance enzymatic activity of poly (ethylene terephthalate) hydrolysis, Nature Catalysis, 2021, 4, 425-430 (Highlighted by ChemistryViews, 科技日报, 中国科学报)
20. Chen, C.-C.,# Yu, X.,# Kuo, C.-J.,# Min, J., Chen, S., Ma, L.,* Liu, K.,* Guo, R.-T.*. Overview of antiviral drug candidates targeting coronaviral 3C-like main proteases. FEBS Journal, 2021, in press. (Review, https://doi.org/10.1111/febs.15696)
21. Chen, C.-C., Min, J., Zhang, L., Yang, Y., Yu, X.,* Guo, R.-T.*. Advanced Understanding of the Electron Transfer Pathway of Cytochrome P450s. ChemBioChem, 2021, 22, 1317-1328 (Review, Cover story)
22. Chen, C.-C.,# Malwal, S. R.,# Han, X.,# Liu, W., Ma, L., Zhai, C., Dai, L., Huang, J.-W., Shillo, A., Desai, J., Ma, X., Zhang, Y., Guo, R.-T.,* Oldfield, E.*. Terpene Cyclases and Prenyltransferases: Structures and Mechanisms of Action. ACS Catalysis, 2021, 11, 290-303 (Cover story)
23. Zhang, L.,# Xie, Z.,# Liu, Z.,# Zhou, S., Ma, L., Liu, W., Huang, J.-W., Ko, T.-P., Li, X., Hu, Y., Min, J., Yu, X., Guo, R.-T.,* Chen, C.-C.*. Structural insight into the electron transfer pathway of a self-sufficient P450 monooxygenase. Nature Communications, 2020, 11, 2676. (Highlighted by C&EN, ChemistryViews, 科技日报, 中国科学报)
24. Chen, C.-C.,* Zhang, L., Yu, X., Ma, L., Ko, T.-P., Guo, R.-T.*. Versatile cis-isoprenyl Diphosphate Synthase Superfamily Members in Catalyzing Carbon–Carbon Bond Formation. ACS Catalysis, 2020, 10, 4717-4725 (Review, ACS Editors’ Choice, Cover story)
25. Chen, C.-C., Dai, L., Ma, L.,* Guo, R.-T.*. Enzymatic degradation of plant biomass and synthetic polymers Nature Reviews Chemistry, 2020, 4, 114-126 (Review, Cover story)
26. Huang, J.-W.,# Niu, D.,# Liu, K.,# Wang, Q., Ma, L., Chen, C.-C., Zhang, L., Liu, W., Zhou, S., Min, J., Wu, S., Yang, Y.,* Guo, R.-T.*. Structure basis of non-structural protein pA151R from African Swine Fever Virus. Biochemical and Biophysical Research Communications, 2020, 532, 108-113.
27. Liu, Y., Lee, C., Li, F., Trček, J., Bähre, H., Guo, R.-T., Chen, C.-C., Chernobrovkin, A., Zubarev, R., Römling, U.*. A Cyclic di-GMP Network Is Present in Gram-Positive Streptococcus and Gram-Negative Proteus Species. ACS Infectious Diseases, 2020, 6, 2672-2687.
28. Xiao, X., Elsayed, S. S., Wu, C., van der Heul, H. U., Metsä-Ketelä, M., Du, C., Prota, A. E., Chen, C.-C., Liu, W., Guo, R.-T., Abrahams, J. P., van Wezel, G. P.*. Functional and Structural Insights into a Novel Promiscuous Ketoreductase of the Lugdunomycin Biosynthetic Pathway. ACS Chemical Biology, 2020, 15, 2529-2538
29. Zhou, S., Ko, T.-P.,* Huang, J.-W., Liu, W., Zheng, Y., Wu, S., Wang, Q., Xie, Z., Liu, Z., Chen, C.-C., * Guo, R.-T.*. Structure of a gut microbial Diltiazem-metabolizing enzyme suggests possible substrate binding mode. Biochemical and Biophysical Research Communications, 2020, 527, 799-804.
30. Yang, Y., Li, L., Yuan, L., Zhou, X., Duan, J., Xiao, H., Cai, N., Han, S., Ma, X., Liu, W., Chen, C.-C., Wang, L., Li, X., Chen, J., Kang, N., Chen, J., Shen, Z., Malwal, S. R., Liu, W., Shi, Y., Oldfield, E., Guo, R.-T., Zhang, Y. A Structural change in butyrophilin upon phosphoantigen binding underlies phosphoantigen-mediated Vgamma9Vdelta2 T cell activation. Immunity, 2019, 50, 1043-1053
31. Sun, H., Ko, T.-P., Liu, W., Liu, W., Zheng, Y., Chen, C.-C.,* Guo, R.-T.*. Structure of an antibiotic-synthesizing UDP-glucuronate 4-epimerase MoeE5 in complex with substrate. Biochemical and Biophysical Research Communications, 2019, 521, 31-36.
32. Kuo, C.-J., Gao, J., Huang, J.-W., Ko, T.-P., Zhai, C., Ma, L., Liu, W., Dai, L., Chang, Y.-F., Chen, T.-H., Hu, Y., Yu, X., Guo, R.-T.,* Chen, C.-C.*. Functional and structural investigations of fibronectin-binding protein Apa from Mycobacterium tuberculosis. (2019) Biochimica et Biophysica Acta. General subjects, 2019, 1863, 1351-1359
33. Tang, X.,# Xue, J.,# Yang, Y., Ko, T.-P., Chen, C.-Y., Dai, L., Guo, R.-T., Zhang, Y.,* Chen C.-C.*. Structural insights into the calcium dependence of Stig cyclases. RSC Advances, 2019, 9, 13182.
34. Ma, J., Ko, T.-P., Yu, X., Zhang, L., Ma, L., Zhai, C., Guo, R.-T., Liu, W., Li, H., Chen, C.-C.*. Structural insights to heterodimeric cis-prenyltransferases through yeast dehydrodolichyl diphosphate synthase subunit Nus1. Biochemical and Biophysical Research Communications, 2019, 515, 621-626.
35. Zhang, L., Ko, T.-P., Malwal S. R., Liu W., Zhou S., Oldfield E., Guo R. T.,* and Chen, C.-C.*. Complex structures of MoeN5 with substrate analogues suggest sequential catalytic mechanism, Biochemical and Biophysical Research Communications, 2019, 511, 800-805.
36. Chang, Z., Ansbacher, T., Zhang, L., Yang, Y., Ko, T.-P., Zhang, G., Liu, W., Huang, J.-W., Dai, L., Guo, R.-T., Major, D. T.,* Chen, C.-C.*. Crystal structure of LepI, a multifunctional SAM-dependent enzyme which catalyzes pericyclic reactions in leporin biosynthesis, Organic Biomolecular Chemistry, 2019, 17, 2070-2076. (Cover story)
37. Chen, C.-C.,# Hu, X.,# Tang, X.,# Yang, Y.,# Ko, T.-P., Gao, J., Zheng, Y., Huang, J.-W., Yu, Z., Li, L., Han, S., Cai, N., Zhang, Y.,* Liu, W.,* Guo, R.-T.*. The Crystal Structure of a Class of Cyclases that Catalyze the Cope Rearrangement. Angewandte Chemie International Edition, 2018, 57, 15060-15064. (Cover story)
38. Malwal, S. R., Chen, L., Hicks, H., Qu, F., Liu, W., Shillo, A., Law, W. X., Zhang, J., Chandnani, N., Han, X., Zheng, Y., Chen, C.-C., Guo, R.-T., AbdelKhalek, A., Seleem, M. N., Oldfield, E. Discovery of lipophilic bisphosphonates that target bacterial cell wall and quinone biosynthesis. Journal of Medicinal Chemistry, 2019, 62, 2564-2581.
39. Gao, J.,# Ko, T.-P.,# Chen, L.,# Malwal, S. R., Zhang, J., Hu, X., Qu, F., Liu, W., Huang, J.-W., Cheng, Y. S., Chen, C.-C., Yang, Y., Zhang, Y., Oldfield, E., Guo, R.-T.*. “Head-to-middle” and “head-to-tail” cis-prenyl transferases: Structure of isosesquilavandulyl diphosphate synthase. Angewandte Chemie International Edition, 2018, 57, 683-687. (Cover story)
40. Chen, C.-C.,# Han, X.,# Ko, T.-P., Liu, W.,* Guo, R.-T.*. Structural studies reveal the molecular mechanism of PETase. FEBS Journal, 2018, 285, 3717-3723.
41. You, S., Chen, C.-C., Tu, T., Wang, X., Ma, R., Cai, H. Y., Guo, R.-T.,* Luo, H. Y.,* Yao, B.*. Insight into the functional roles of Glu175 in the hyperthermostable xylanase XYL10C-ΔN through structural analysis and site-saturation mutagenesis. Biotechnol Biofuels, 2018, 11, 159.
42. Zheng, Y.,# Liu, W.,# Chen C.-C.,# Hu, X., Liu, W., Ko, T.-P., Tang, X., Wei, H., Huang, J.-W., Guo, R.-T.*. Crystal Structure of a Mycoestrogen-Detoxifying Lactonase from Rhinocladiella mackenziei: Molecular Insight into ZHD Substrate Selectivity. ACS Catalysis. 2018, 8, 4294-4298.
43. Malwal, S. R.,# Gao, J.,# Hu, X.,# Yang, Y.,# Liu, W., Huang J.-W., Ko, T.-P., Li, L., Chen, C.-C., O’Dowd, B., Khade, R. L., Zhang, Y., Zhang, Y., Oldfield, E., Guo, R.-T.*. Catalytic role of conserved asparagine, glutamine, serine, and tyrosine residues in isoprenoid biosynthesis enzymes. ACS Catalysis. 2018, 8, 4299-4312.
44. Wang, J.,# Chen, C.-C.,# Yang, Y.,# Liu, W., Ko, T.-P., Shang, N., Hu, X., Xie, Y., Huang, J.-W., Zhang, Y.,* Guo, R.-T.*. Structural insight into a novel indole prenyltransferase in hapalindole-type alkaloid biosynthesis. Biochemical and Biophysical Research Communications, 2018, 495, 1782-1788.
45. Han, X.,# Liu, W.,# Huang, J.-W.,# Ma, J., Zheng, Y., Ko, T.-P., Xu, L., Cheng, Y. S., Chen, C.-C., Guo, R.-T.*. Structural insight into catalytic mechanism of PET hydrolase. Nature Communications, 2017, 13, 2106.
46. Gao, J.,# Huang, J.-W.,# Li, Q.,# Liu, W., Ko, T.-P., Zheng, Y., Xiao, X., Kuo, C.-J., Chen, C.-C.,* Guo, R.-T.*. Characterization and crystal structure of a thermostable glycoside hydrolase family 45 1,4-β-endoglucanase from Thielavia terrestris. Enzyme and Microbial Technology, 2017, 99, 32-37.
47. Hui, R.,# Hu, X.,# Liu, W., Zheng, Y., Chen, Y., Guo, R.-T., Jin, J.,* Chen, C.-C.*. Characterization and crystal structure of a novel zearalenone hydrolase from Cladophialophora bantiana. Acta Crystallographica. Section F, Structural Biology Communications, 2017, 73, 515-519.
48. Zhang, L.,# Chen, C.-C.,# Ko, T.-P.,# Huang, J.-W., Zheng, Y., Liu, W., Wang, I., Malwal, S. R., Feng, X., Wang, K., Huang, C.-C., Hsu, S.-T., Wang, A.-H., Oldfield, E.,* and Guo, R.-T.*. Moenomycin Biosynthesis: Structure and Mechanism of Action of the Prenyl Transferase MoeN5, Angewandte Chemie International Edition, 2016, 55, 4716-4720.
49. Xu, Z.,# Liu, W.,# Chen, C.-C.,# Li Q., Huang, J.-W., Ko, T.-P., Liu, G., Liu, W., Peng, W., Cheng, Y.-S., Chen, Y., Jin, J., Li, H.,* Zheng, Y.,* Guo, R.-T.*. Enhanced α‑Zearalenol Hydrolyzing Activity of a Mycoestrogen Detoxifying Lactonase by Structure-Based Engineering. ACS Catalysis, 2016, 6, 7657-7663. (Cover story)
50. Liu, M.,# Chen, C.-C.,# Chen, L.,# Xiao, X., Zheng, Y.,Huang, J.-W., Liu, W., Ko, T.-P., Cheng, Y.-S., Feng, X., Oldfield, E.,* Guo, R.-T.,* and Ma, Y.*. Structure and Function of a “Head-to-Middle” Prenyltransferase: Lavandulyl Diphosphate Synthase, Angewandte Chemie International Edition, 2016, 55, 4721-4724. (Cover story)
51. Yan, J.,# Liu, W.,# Li, Y., Lai, H.-L., Zheng, Y., Huang, J.-W., Chen, C.-C., Chen, Y., Jin, J., Li, H.,* Guo, R.-T.*. Functional and structural analysis of Pichia pastoris expressed Aspergillus niger 1,4-β-endoglucanase. Biochemical and Biophysical Research Communications, 2016, 17, 8-12.
52. Huang, J.-W.,# Liu, W.,# Lai, H.-L., Cheng, Y.-S., Zheng, Y., Li, Q., Sun, H., Kuo, C.-J., Guo, R.-T.,* Chen, C.-C.*. Crystal structure and genetic modifications of FI-CMCase from Aspergillus aculeatus F-50. Biochemical and Biophysical Research Communications, 2016, 16, 565-572.
53. Yang, Y.,# Ko, T.-P.,# Chen, C.-C.,# Huang, G., Zheng, Y., Liu, W., Wang, I., Ho, M., Hsu, S.-T., O'Dowd, B., Huff, H., Huang, C.-H., Docampo, R., Oldfield, E.,* Guo, R.-T.,*. Structures of Trypanosome Vacuolar Soluble Pyrophosphatases: Anti-Parasitic Drug Targets. ACS Chemical Biology, 2016, 11, 1362-1371.
54. Zheng, Y.,# Li, Y.,# Liu, W.,# Chen, C.-C., Ko, T.-P., He, M., Xu, Z., Liu, M., Luo, H., Guo, R.-T.,* Yao, B.,* and Ma, Y.*. Structural insight into potential cold adaptation mechanism through a psychrophilic glycoside hydrolase family 10 endo-β-1, 4-xylanase. Journal of Structural Biology, 2016, 193, 206-211. (Cover story)
55. Liu, G., Li, Q., Shang, N., Huang J. W., Ko, T.-P., Liu, W., Zheng, Y., Han, X., Chen, Y., Chen, C.-C., Jin, J., Guo, R.-T.*. Functional and structural analyses of a 1,4- β-endoglucanase from Ganoderma lucidum. Enzyme and Microbial Technology, 2016, 86, 67-74.
56. Hu, Y.,# Liu, W.,# Malwal, S. R., Zheng, Y., Feng, X., Ko, T.-P., Chen, C.-C., Xu, Z., Liu, M., Han, X., Gao, J., Oldfield, E.,* Guo, R.-T.*. Structures of iridoid synthase from Cantharanthus roseus with bound NAD(+), NADPH, or NAD(+) /10-oxogeranial: Reaction mechanisms. Angewandte Chemie International Edition, 2015, 54, 15478-15482. (Cover story)
57. Haywood, J., Qi, J., Chen, C.-C., Lu, G., Liu, Y., Yan, J., Shi, Y., and Gao, G.-F. Structural basis of collagen recognition by human osteoclast-associated receptor and design of osteoclastogenesis inhibitors, PNAS, 2016, 113, 1038-1043.
58. Cheng, Y.-S.,# Chen, C.-C.,# Huang, J.-W., Ko, T.-P., Huang, Z., and Guo, R.-T.*. Improving the catalytic performance of a GH11 xylanase by rational protein engineering, Applied Microbiology and Biotechnology, 2015, 99, 9503-9510.
59. Zheng, Y.,# Chen, C.-C.,# Ko, T.-P., Xiao, X., Yang, Y., Huang, C.-H., Qian, G., Shao, W.,* and Guo, R.-T.*. Crystal structures of S-adenosyl homocysteine hydrolase from the thermophilic bacterium Thermotoga maritima, Journal of Structural Biology, 2015, 190, 135-142. (Cover story)
60. Chen, C.-C., Cheng, K.-J., Ko, T.-P., and Guo, R.-T.*. Current progresses in phytase research: Three-Dimensional Structure and Protein Engineering, ChemBioEng Reviews, 2015, 2, 76-86. (Review, Cover story)
61. Chen, C.-C., Ko, T.-P., Huang, J.-W., and Guo, R.-T.*. Heat- and alkaline-stable xylanases: applications, protein structure and engineering, ChemBioEng Reviews, 2015, 2, 95-106. (Review)
62. Chen, Y., Huang, J.-W., Chen, C.-C., Lai, H.-L., Jin, J., Guo, R.-T.*. Crystallization and preliminary X-ray diffraction analysis of an endo-1, 4-beta-D-glucanase from Aspergillus aculeatus F-50. Acta Crystallographica. Section F, Structural Biology Communications, 2015, 71, 397-400.
63. Zhu, W., Wang, Y., Li, K., Gao, J., Huang, C.-H., Chen, C.-C., Ko, T.-P., Zhang, Y., Guo, R.-T., Oldfield, E.*. Antibacterial drug leads: DNA and enzyme multi-targeting. Journal of Medicinal Chemistry, 2015, 58, 1215-1227.
64. Chen, C.-C.,# Huang, J.-W.,# Zhao, P., Ko, T.-P., Huang, C.-H., Chan, H.-C., Huang, Z., Liu, W., Cheng, Y.-S., Liu, J.-R., and Guo, R.-T.*. Structural analyses and yeast production of the β-1,3-1,4-glucanase catalytic module encoded by the licB gene of Clostridium thermocellum, Enzyme and Microbial Technology, 2015, 71, 1-7.
65. Han, X.,# Chen, C.-C.,# Kuo, C.-J., Huang, C.-H., Zheng, Y., Ko, T.-P., Zhu, Z., Feng, X., Wang, K., Oldfield, E., Wang, A.-H., Liang, P.-H., Guo, R.-T.,* and Ma, Y.*. Crystal structures of ligand-bound octaprenyl pyrophosphate synthase from Escherichia coli reveal the catalytic and chain-length determining mechanisms, Proteins, 2015, 83, 37-45.
66. Cheng, Y.-S.,# Chen, C.-C.,# Huang, C.-H., Ko, T.-P., Luo, W., Huang, J.-W., Liu, J.-R., and Guo, R.-T.*. Structural analysis of a glycoside hydrolase family 11 xylanase from Neocallimastix patriciarum: insights into the molecular basis of a thermophilic enzyme, The Journal of Biological Chemistry, 2014, 289, 11020-11028.
67. Liu, W.,# Feng, X.,# Zheng, Y.,# Huang, C. H., Nakano, C., Hoshino, T., Bogue, S., Ko, T.-P., Chen, C.-C., Cui, Y., Li, J., Wang, I., Hsu, S. T., Oldfield, E.,* Guo, R.-T.*. Structure, function and inhibition of ent-kaurene synthase from Bradyrhizobium japonicum. Scientific Reports, 2014, 4, 6214.
68. Shang, N.,# Li, Q.,# Ko, T.-P.,# Chan, H.-C., Li, J., Zheng, Y., Huang, C.-H., Ren, F., Chen, C.-C., Zhu, Z., Galizzi, M., Li, Z.-H., Rodrigues-Poveda, C. A., Gonzalez-Pacanowska, D., Veiga-Santos, P., de Carvalho, T. M., de Souza, W., Urbina, J. A., Wang, A.-H., Docampo, R., Li, K., Liu, Y.-L., Oldfield, E.,* Guo, R.-T.*. Squalene synthase as a target for Chagas disease therapeutics. PLoS Pathogens, 2014, 10, e1004114.
69. Peng, W.,# Ko, T.-P.,# Yang, Y., Zheng, Y., Chen, C.-C., Zhu, Z., Huang, C.-H., Zeng, Y.-F., Huang, J.-W., Wang, A. H.-J., Liu, J.-R.,* Guo, R.-T.*. Crystal structure and substrate-binding mode of the mycoestrogen-detoxifying lactonase ZHD from Clonostachys rosea. RSC Advances, 2014, 4, 62321-62325.
70. Huang, C.-H., Zhu, Z., Cheng, Y.-S., Chan, H.-C., Ko, T.-P., Chen, C.-C., Wang, I., Ho, M.-R., Hsu, S. T. -D., Zeng, Y.-F., Huang, Y.-N., Liu, J.-R.,* Guo, R.-T.*. Structure and Catalytic Mechanism of a Glycoside Hydrolase Family-127β-L-Arabinofuranosidase (HypBA1). J Bioprocess Biotech, 2014, 4, 171.
71. Zhang, L.,# Zhao, P.,# Chen, C.-C., Huang, C.-H., Ko, T.-P., Zheng, Y., Guo, R.-T.*. Preliminary X-ray diffraction analysis of a thermophilic beta-1,3-1,4-glucanase from Clostridium thermocellum. Acta Crystallographica. Section F, Structural Biology Communications, 2014, 70, 946-948.
72. Xu, J.,# Ren, F.,# Huang, C.-H., Zheng, Y., Zhen, J., Sun, H., Ko, T.-P., He, M., Chen, C.-C., Chan, H.-C., Guo, R.-T.,* Song, H.,* Ma, Y.*. Functional and structural studies of pullulanase from Anoxybacillus sp. LM18-11. Proteins, 2014, 82, 1685-1693. (Cover story)
73. Wu, T.-H.,# Chen, C.-C.,# Cheng, Y.-S., Ko, T.-P., Lin, C.-Y., Lai, H.-L., Huang, T.-Y., Liu, J.-R., * and Guo, R.-T.*. Improving specific activity and thermostability of Escherichia coli phytase by structure-based rational design, Journal of Biotechnology, 2014, 175, 1-6.
74. Lv, P.,# Zhang, L.,# Luo, H., Chen, C.-C., Huang, C.-H., Peng, W., Wang, K., Ko, T.-P., Zheng, Y., Zhang, J., Yao, B.,* Guo, R.-T.*. Preliminary X-ray diffraction analysis of thermostable beta-1,4-xylanase from Streptomyces sp. S9. Acta Crystallographica. Section F, Structural Biology Communications, 2014, 70, 105-107.
75. Huang, J.-W.,# Chen, C.-C.,# Huang, C.-H., Huang, T.-Y., Wu, T.-H., Cheng, Y.-S., Ko, T.-P., Lin, C.-Y., Liu, J.-R.,* and Guo, R.-T.,*. Improving the specific activity of beta-mannanase from Aspergillus niger BK01 by structure-based rational design, Biochimica et Biophysica Acta, 2014, 1844, 663-669. (Cover story)
76. Cheng, Y.-S.,# Huang, C.-H.,# Chen, C.-C., Huang, T.-Y., Ko, T.-P., Huang, J.-W., Wu, T.-H., Liu, J.-R.,* Guo, R.-T.,*. Structural and mutagenetic analyses of a 1,3-1,4-beta-glucanase from Paecilomyces thermophila. Biochimica et Biophysica Acta, 2014, 1844, 366-373. (Cover story)
77. Chen, C.-C.,# Luo, H.,# Han, X.,# Lv, P., Ko, T.-P., Peng, W., Huang, C.-H., Wang, K., Gao, J., Zheng, Y., Yang, Y., Zhang, J., Yao, B.,* and Guo, R.-T.*. Structural perspectives of an engineered beta-1,4-xylanase with enhanced thermostability, Journal of Biotechnology, 2014, 189, 175-182.
78. Ren, F.,# Feng, X.,# Ko, T.-P.,# Huang, C.-H., Hu, Y., Chan, H.-C., Liu, Y.-L., Wang, K., Chen, C.-C., Pang, X., He, M., Li, Y., Oldfield, E.,* Guo, R.-T.*. Insights into TIM-barrel prenyl transferase mechanisms: crystal structures of PcrB from Bacillus subtilis and Staphylococcus aureus. ChemBioChem : a European Journal of Chemical Biology, 2013, 14, 195-199. (Cover story)
79. Luo, W.,# Huang, J.-W.,# Huang, C.-H., Huang, T.-Y., Chan, H.-C., Liu, J.-R., Guo, R.-T., Chen, C.-C.*. Preliminary X-ray diffraction analysis of thermostable beta-1,4-mannanase from Aspergillus niger BK01. Acta Crystallographica. Section F, Structural Biology and Crystallization Communications, 2013, 69, 1100-1102.
80. Li, X.,# Han, X.,# Ko, T.-P., Chen, C.-C., Zhu, Z., Hua, E., Guo, R.-T., Huang, C.-H.*. Preliminary X-ray diffraction analysis of octaprenyl pyrophosphate synthase from Escherichia coli. Acta crystallographica. Section F, Structural Biology and Crystallization Communications, 2013, 69, 328-331.
81. Han, X.,# Gao, J.,# Shang, N.,# Huang, C.-H., Ko, T.-P., Chen, C.-C., Chan, H.-C., Cheng, Y.-S., Zhu, Z., Wiegel, J., Luo, W.,*Guo, R.-T.,* Ma, Y.*. Structural and functional analyses of catalytic domain of GH10 xylanase from Thermoanaerobacterium saccharolyticum JW/SL-YS485. Proteins, 2013, 81, 1256-1265. (Cover story)
82. Lin, C.-W., Chang, Y.-L., Chang, Y.-C., Lin, J.-C., Chen, C.-C., Pan, S.-H., Wu, C.-T., Chen, H.-Y., Yang, S.-C., Hong, T.-M., and Yang, P.-C. MicroRNA-135b promotes lung cancer metastasis by regulating multiple targets in the Hippo pathway and LZTS1, Nature communications, 2013, 4, 1877.
83. Huang, J.-W., Cheng, Y.-S., Ko, T.-P., Lin, C.-Y., Lai, H.-L., Chen, C.-C., Ma, Y., Zheng, Y., Huang, C.-H., Zou, P., Liu, J.-R.,* Guo, R.-T.*. Rational design to improve thermostability and specific activity of the truncated Fibrobacter succinogenes 1,3-1,4-beta-D-glucanase. Applied Microbiology and Biotechnology, 2012, 94, 111-121.
84. Huang, C.-H.,# Sun, Y.,# Ko, T.-P., Chen, C.-C., Zheng, Y., Chan, H.-C., Pang, X., Wiegel, J.,* Shao, W.,* Guo, R.-T.*. The substrate/product-binding modes of a novel GH120 beta-xylosidase (XylC) from Thermoanaerobacterium saccharolyticum JW/SL-YS485. The Biochemical Journal, 2012, 448, 401-407.
85. Chan, H.-C.,# Zhu, Y.,# Hu, Y., Ko, T.-P., Huang, C.-H., Ren, F., Chen, C.-C., Ma, Y., Guo, R.-T.,* Sun, Y.*. Crystal structures of D-psicose 3-epimerase from Clostridium cellulolyticum H10 and its complex with ketohexose sugars. Protein & Cell, 2012, 3, 123-131. (Cover story)
86. Chen, C.-C., Chang, C.-M., Sun, C.-P., Yu, C.-P., Wu, P.-Y., Jeng, K.-S., Hu, C.-P., Chen, P.-J., Wu, J.-C., Shih, C.-H., Gershwin, M. E., and Tao, M.-H. Use of RNA interference to modulate liver adenoma development in a murine model transgenic for hepatitis B virus, Gene therapy, 2012, 19, 25-33.
87. Zeng, Y.-F.,# Ko, T.-P.,# Lai, H.-L., Cheng, Y.-S., Wu, T.-H., Ma, Y., Chen, C.-C., Yang, C.-S., Cheng, K.-J., Huang, C.-H., Guo, R.-T.,* Liu, J.-R.*. Crystal structures of Bacillus alkaline phytase in complex with divalent metal ions and inositol hexasulfate. Journal of Molecular Biology, 2011, 409, 214-224. (Cover story)
88. Wu, T.-H.,# Huang, C.-H.,# Ko, T.-P., Lai, H.-L., Ma, Y., Chen, C.-C., Cheng, Y.-S., Liu, J.-R.,* Guo, R.-T.*. Diverse substrate recognition mechanism revealed by Thermotoga maritima Cel5A structures in complex with cellotetraose, cellobiose and mannotriose. Biochimica et Biophysica Acta, 2011, 1814, 1832-1840. (Cover story)
89. Chen, C.-C., Sun, C.-P., Ma, H.-I., Fang, C.-C., Wu, P.-Y., Xiao, X., and Tao, M.-H. Comparative study of anti-hepatitis B virus RNA interference by double-stranded adeno-associated virus serotypes 7, 8, and 9, Molecular therapy : the journal of the American Society of Gene Therapy, 2009, 17, 352-359.
90. Chen, C.-C., Ko, T.-M., Ma, H.-I., Wu, H.-L., Xiao, X., Li, J., Chang, C.-M., Wu, P.-Y., Chen, C.-H., Han, J.-M., Yu, C.-P., Jeng, K.-S., Hu, C.-P., and Tao, M.-H. Long-term inhibition of hepatitis B virus in transgenic mice by double-stranded adeno-associated virus 8-delivered short hairpin RNA, Gene therapy, 2007, 14, 11-19.
六、已授权专利
1. 具有提升的酶活性的植酸酶，中国，CN104342418B，2/8
2. 具有提升的酶活性的植酸酶，中国，CN106399273B，2/8
3. Cellulase having improved enzymatic activity，美国，US9809807B1，2/8
4. Phytase having improved thermostability，美国，US10011822B2，7/8
5. Glucose oxidase having improved thermostability，美国，US10011822B2，9/10
6. Lactone hydrolase and method of degrading alpha–zearalenol using the same，美国，US10316303B2，4/8
7. Lysozyme having improved enzymatic activity，美国，US10323237B2，1/4
8. Xylosidase having improved enzymatic activity，美国，US20190119687A1，9/10
9. Cellulase having improved enzymatic activity，美国，US16406255，1/11
七、获奖与荣誉称号
1. 2020年，湖北省创新群体
2. 2020年，湖北省省级人才入选者
3. 2019年，天津市自然科学二等奖（第二完成人）
获选的其中16篇封面文章
