一、基本信息
郭瑞庭，二级教授、博士研究生导师
电子邮件：guoreyting(at)hubu.edu.cn
研究方向：酶工程、结构生物学与酶学
研究领域：酶分子结构、机理研究、应用与改造
郭瑞庭教授通过结构生物学方法解析重要工业酶结构，阐释催化机理，进行酶分子理性设计，以改造酶的活性与特性。工作内容主要涵盖五个体系：(1) PET塑料降解酶；(2) 新型萜类合成酶；(3) 霉菌毒素分解酶；(4) 生物质降解酶；(5) P450酶。目前已在Nature Reviews Chemistry, Nature Catalysis, Immunity, Nature Communications, Angew. Chem. Intl. Ed., JACS, ACS Catalysis, PNAS 等SCI期刊发表论文140余篇，超过30篇获选为封面文章，申请国内外专利34个，已授权23个。2019年获天津市自然科学二等奖(第一完成人)。
二、教育背景
2003–2007 台湾大学生化科学所暨台湾中研院生物化学所，博士
2014–2017 台湾政治大学EMBA，获商学硕士
三、工作经历
2018年至今 湖北大学生命科学院、省部共建生物催化与酶工程国家重点实验室，二级教授/博导
2010–2018 中科院天津工业生物技术研究所，研究员、博导
2007–2017 年台湾基酵生物科技公司，研发总监/研发总顾问
2007–2009 美国The Scripps Research Institute，博士后研究
四、科研项目
1. 国家重大专项、2019.12–2024.11，552万、在研、主持
2. 湖北省创新群体、2020.03–2023.03，50万、在研、主持
3. 自然科学基金面上项目（两次），2015.001–2018.012、2019.001-2022.012、85万、在研、主持
4. 863计划、2012.8-2015.12、项目9450万、已结题、项目首席科学家
5. 973计划、2011.01-2015.08、75万、已结题、主持
6. 中科院创新交叉团队、2017.01-2019.12、100万、已结题、团队负责人
7. 中科院重点部署项目，2013.01-2015.12、235万、已结题、主持
8. 中科院百人计划、2010年-2013年、260万、已结题、主持
9. 天津市科技项目、2011年-2014年、150万、已结题、主持
10. 企业合作开发项目 (东莞泛亚太)、2012年-2015年、255万、已结题、主持
四、代表性论文
1. Zhang, X.,# Shen, P.,# Zhao, J.,# Chen, Y., Li, X., Huang, J.-W., Zhang, L., Li, Q., Gao, C., Xing, Q., Chen, C.-C., Guo, R.-T.,* Li, A.*. Rationally controlling selective steroid hydroxylation via scaffold sampling of a P450 Family. ACS Catalysis, 2023, 13, 1280–1289.
2. Chen, Q.,# Li, B.,# Zhang, L.,# Chen, X., Zhu, X., Chen, F., Shi, M., Chen, C.-C., Yang, Y., Guo, R.-T.,* Liu, W., Xu, J., Zheng, G.*. Engineered imine reductase for larotrectinib intermediate manufacture. ACS Catalysis, 2022, 12 (23), 14795-14803.
3. Dai, L.,# Li, H.,# Huang, J.-W.,# Hu, Y.,# He, M., Yang, Y., Min, J., Guo, R.-T.,* Chen, C.-C.*. Structure-based rational design of a short-chain dehydrogenase/reductase for improving activity toward mycotoxin patulin. International Journal of Biological Macromolecules, 2022-12-01, 222: 421-428.
4. Wu, L.,# An, J.,# Jing, X.,# Chen, C.-C., Dai, L., Liu, W.,* Guo, R.-T.,* Nie, Y.*. Molecular insights into the regioselectivity of the Fe(II)/2-ketoglutarate-dependent dioxygenase-catalyzed C-H hydroxylation of amino acids. ACS Catalysis, 2022-09-08, 12: 11586-11596.
5. Zhang, L.,# Zhang, X.,# Min, J.,# Liu, B., Huang, J.-W., Yang, Y., Liu, W., Dai, L., Yang, Y., Chen, C.-C.,* Guo, R.-T.*.. Structural insights to a bi-functional isoprenyl dihposphate synthase that can catalyze head-to-tail and head-to-middle condensation. International Journal of Biological Macromolecules, 2022-08-01, 214: 492-499.
6. Li, H.,# Yang, Y.,# Hu, Y.,# Chen, C.-C., Huang, J.-W., Min, J., Dai, L.,* Guo, R.-T.*. Structural analysis and engineering of aldo-keto reductase from glyphosate-resistant Echinochloa colona. Journal of Hazardous Materials, 2022-08-15, 436: 129191.
7. Malwal, S. R.,# Shang, N.,# Liu, W., Li, X., Zhang, L., Chen, C.-C., Guo, R.-T.,* Oldfield, E.*. A structural and bioinformatics investigation of a fungal squanlene synthase and comparisons with other membrane proteins. ACS Omega, 2022-06-17, 7 (26): 22601-22612.
8. Wang, T.,# Yang, Y.,# He, M., Liu, M., Huang, J.-W., Min, J., Chen, C.-C., Liu, Y.,* Zhang, L.,* Guo, R.-T.*. Structural insights into the cyclization of unusual brasilane-type sesquiterpenes. International Journal of Biological Macromolecules, 2022-04-30, 209: 1784-1791.
9. Dai, L.,# Zhang, X.,# Hu, Y.,# Shen, J., Zhang, Q., Zhang, L., Min, J., Chen, C.-C., Liu, Y.,* Huang, J.-W.,* Guo, R.-T.*. Structural and functional insights into a nonheme iron- and α-ketoglutarate-dependent halogenase that catalyzes chlorination of nucleotide substrates. Applied and Environmental Microbiology, 2022-04-18, 88 (9): e0249721.
10. Zeng, W.,# Li, X.,# Yang, Y.,# Min, J.,# Huang, J.-W., Liu, W., Niu, D., Yang, X., Han, X., Zhang, L., Dai, L., Chen, C.-C.,* Guo, R.-T.*. Substrate-binding mode of a thermophilic PET hydrolase and engineering the enzyme to enhance the hydrolytic efficacy, ACS Catalysis, 2022-02-18, 12: 3033-3040.
11. Chen, C.-C.,# Dai, M.,# Zhang, L.,# Zhao, J., Zeng, W., Shi, M., Huang, J.-W., Liu, W., Guo, R.-T.,* Li, A.*. Molecular basis for a toluene monooxygenase to govern substrate selectivity, ACS Catalysis, 2022-02-13, 12: 2831-2839.
12. Mao, Z.,# Xiao, H.,# Shen, P.,# Yang,Y.,# Xue, J., Yang, Y., Shang, Y., Zhang, L., Li, X., Zhang, Y., Du, Y., Chen, C.-C., Guo, R.-T.,* Zhang, Y.*. KRAS(G12D) can be targeted by potent inhibitors via formation of salt bridge. Cell Discovery, 2022-01-25, 8 (1): 5.
13. Hu, Y.#, Li, H.,# Min, J.,# Yu, Y., Liu, W., Huang, J.-W., Zhang, L., Yang, Y., Dai, L.*, Chen, C.-C.,* Guo, R.-T.*. Crystal structure and biochemical analysis of the specialized deoxynivalenol–detoxifying glyoxalase SPG from Gossypium hirsutum. International Journal of Biological Macromolecules, 2022-01-18, 200: 388-396.
14. 陈纯琪, 韩旭, 刘卫东, 马立新, 刘珂*，郭瑞庭*. 基于结构改造来源于大阪伊德氏杆菌 201-F6 的PET水解酶，生物工程学报，2021-09-25, 37 (9): 3268-3275.
15. Dai, L.,# Chang, Z.,# Yang, J., Liu,W., Yang, Y., Chen, C.-C., Zhang, L., Huang, J.-W.,* Sun, Y.,* Guo, R.-T.*. Structural investigation of a thermostable 1,2- β -mannobiose phosphorylase from Thermoanaerobacter sp. X-514. Biochemical and Biophysical Research Communications, 2021-09-21, 579: 54-61.
16. Dai, L.,# Qu, Y.,# Hu, Y.,# Min, J., Yu, X., Chen, C.-C., Huang, J.-W.,* Guo, R.-T.*. Catalytically inactive lytic polysaccharide monooxygenase PcAA14A enhances the enzyme-mediated hydrolysis of polyethylene terephthalate, International Journal of Biological Macromolecules, 2021-09-07, 190: 456-462.
17. Dai, L.,# Qu, Y.,# Huang, J.-W., Hu, Y., Hu, H., Li, S., Chen, C.-C.,* Guo, R.-T.*. Enhancing PET hydrolytic enzyme activity by fusion of the cellulose-binding domain of cellobiohydrolase I from Trichoderma reesei. Journal of Biotechnology, 2021-05-24, 334: 47-50.
18. Liu, W.,# Ma, C.,# Liu, W.,# Zheng, Y., Chen, C.-C., Liang, A., Luo, X., Li, Z., Ma, W., Song, Y.,* Guo, R.-T.,* Zhang, T.*. Functional and structural investigation of a novel β-mannanase BaMan113A from Bacillus sp. N16-5. International Journal of Biological Macromolecules, 2021-04-15, 182: 899-909.
19. Chen, C.-C.,# Han, X.,# Li, X.,# Jiang, P., Niu, D., Ma, L., Liu, W., Li, S., Qu, Y., Hu, H., Min, J., Yang, Y., Zhang, L., Zeng, W., Huang, J.-W.,* Dai, L.,* Guo, R.-T.* General features to enhance enzymatic activity of poly (ethylene terephthalate) hydrolysis, Nature Catalysis, 2021, 4, 425-430 (Highlighted by ChemistryViews, 科技日报, 中国科学报)
20. Dai, L., Hu, Y., Chen, C.-C., Ma, L.,* Guo, R.-T.* Flavonoid C-glycosyltransferases: function, evolutionary relationship, catalytic mechanism and protein engineering, ChemBioEng Reviews, 2021-01-20, 21 (1): 15-26.
21. Chen, C.-C.,# Yu, X.,# Kuo, C.-J.,# Min, J., Chen, S., Ma, L.,* Liu, K.,* Guo, R.-T.* Overview of antiviral drug candidates targeting coronaviral 3C-like main proteases. FEBS Journal, 2021, (Review, https://doi.org/10.1111/febs.15696)
22. Chen, C.-C., Min, J., Zhang, L., Yang, Y., Yu, X.,* Guo, R.-T.* Advanced Understanding of the Electron Transfer Pathway of Cytochrome P450s. ChemBioChem, 2021, 22, 1317-1328 (Review, Cover story)
23. Chen, C.-C.,# Malwal, S. R.,# Han, X.,# Liu, W., Ma, L., Zhai, C., Dai, L., Huang, J.-W., Shillo, A., Desai, J., Ma, X., Zhang, Y., Guo, R.-T.,* Oldfield, E.* Terpene Cyclases and Prenyltransferases: Structures and Mechanisms of Action. ACS Catalysis, 2021, 11, 290-303 (Cover story)
24. Chen, C.-C.,# Jian Min, J.,# Zhang, L., Yang, Y., Yu, X.,* Guo, R.-T.* Advanced understanding of electron transfer pathway of cytochrome P450s, ChemBioChem，2021-07-05, 22: 1317-1328. (Review, Cover story)
25. Chen, C.-C., Xue, J., Peng, W., Wang, B., Zhang, L., Liu, W., Ko, T.-P., Huang, J.-W., Zhou, S., Min, J., Ma, L., Dai, L.,* Guo, R.-T.*. Yu, X.*. Structural insights into thebaine synthase 2 catalysis. Biochemical and Biophysical Research Communications, 2020-06-22, 529 (2):156-161.
26. Zhang, L.,# Xie, Z.,# Liu, Z.,# Zhou, S., Ma, L., Liu, W., Huang, J.-W., Ko, T.-P., Li, X., Hu, Y., Min, J., Yu, X., Guo, R.-T.,* Chen, C.-C.*. Structural insight into the electron transfer pathway of a self-sufficient P450 monooxygenase. Nature Communications, 2020, 11, 2676. (Highlighted by C&EN, ChemistryViews, 科技日报, 中国科学报)
27. Chen, C.-C.,* Zhang, L., Yu, X., Ma, L., Ko, T.-P., Guo, R.-T.*. Versatile cis-isoprenyl diphosphate synthase superfamily members in catalyzing carbon–carbon bond formation. ACS Catalysis, 2020, 10, 4717-4725. (Review, ACS Editors’ Choice, Cover story)
28. Chen, C.-C., Dai, L., Ma, L.,* Guo, R.-T.*. Enzymatic degradation of plant biomass and synthetic polymers. Nature Reviews Chemistry, 2020, 4, 114-126. (Review, Cover story)
29. Song, M., Zhang, X., Liu, W.,* Feng, J.,* Cui, Y., Yao, P., Wang, M., Guo, R.-T., Wu, Q., Zhu, D.*. 2,3-Dihydroxybenzoic Acid Decarboxylase from Fusarium oxysporum: Crystal Structures and Substrate Recognition Mechanism. ChemBioChem, 2021, 21, 2950-2956.
30. Huang, J.-W.,# Niu, D.,# Liu, K.,# Wang, Q., Ma, L., Chen, C.-C., Zhang, L., Liu, W., Zhou, S., Min, J., Wu, S., Yang, Y.,* Guo, R.-T.*. Structure basis of non-structural protein pA151R from African Swine Fever Virus. Biochemical and Biophysical Research Communications, 2020, 532, 108-113.
31. Liu, Y., Lee, C., Li, F., Trček, J., Bähre, H., Guo, R.-T., Chen, C.-C., Chernobrovkin, A., Zubarev, R., Römling, U. A Cyclic di-GMP Network Is Present in Gram-Positive Streptococcus and Gram-Negative Proteus Species. ACS Infectious Diseases, 2020, 6, 2672-2687.
32. Xiao, X., Elsayed, S. S., Wu, C., van der Heul, H. U., Metsä-Ketelä, M., Du, C., Prota, A. E., Chen, C.-C., Liu, W., Guo, R.-T., Abrahams, J. P., van Wezel, G. P. Functional and Structural Insights into a Novel Promiscuous Ketoreductase of the Lugdunomycin Biosynthetic Pathway. ACS Chemical Biology, 2020, 15, 2529-2538
33. Zhou, S., Ko, T.-P.,* Huang, J.-W., Liu, W., Zheng, Y., Wu, S., Wang, Q., Xie, Z., Liu, Z., Chen, C.-C.,* Guo, R.-T.*. Structure of a gut microbial Diltiazem-metabolizing enzyme suggests possible substrate binding mode. Biochemical and Biophysical Research Communications, 2020, 527, 799-804.
34. Chen, X., Zhang, H., Maria-S., Miguel A., Liu, W., Li, J., Feng, J., Liu, X., Osuna S.,* Guo, R.-T., Wu, Q.,* Zhu, D.,* Ma, Y. Efficient reductive desymmetrization of bulky 1, 3-cyclodiketones enabled by structure-guided directed evolution of a carbonyl reductase, Nature Catalysis, 2019, 2, 931-941.
35. Yang, Y., Li, L., Yuan, L., Zhou, X., Duan, J., Xiao, H., Cai, N., Han, S., Ma, X., Liu, W., Chen, C.-C., Wang, L., Li, X., Chen, J., Kang, N., Chen, J., Shen, Z., Malwal, S. R., Liu, W., Shi, Y., Oldfield, E., Guo, R.-T.,* Zhang, Y.*. A structural change in butyrophilin upon phosphoantigen binding underlies phosphoantigen-mediated Vgamma9Vdelta2 T cell activation. Immunity, 2019, 50, 1043-1053
36. Sun, H., Ko, T.-P., Liu, W., Liu, W., Zheng, Y., Chen, C.-C.,* Guo, R.-T.*. Structure of an antibiotic-synthesizing UDP-glucuronate 4-epimerase MoeE5 in complex with substrate. Biochemical and Biophysical Research Communications, 2019, 521, 31-36.
37. Kuo, C.-J., Gao, J., Huang, J.-W., Ko, T.-P., Zhai, C., Ma, L., Liu, W., Dai, L., Chang, Y.-F., Chen, T.-H., Hu, Y., Yu, X., Guo, R.-T.,* Chen, C.-C.*. Functional and structural investigations of fibronectin-binding protein Apa from Mycobacterium tuberculosis. Biochimica et Biophysica Acta. General subjects, 2019, 1863, 1351-1359
38. Tang, X.,# Xue, J.,# Yang, Y., Ko, T.-P., Chen, C.-Y., Dai, L., Guo, R.T., Zhang, Y.,* Chen C. C.*. Structural insights into the calcium dependence of Stig cyclases. RSC Advances, 2019, 9, 13182.
39. Ma, J., Ko, T.-P., Yu, X., Zhang, L., Ma, L., Zhai, C., Guo, R.-T., Liu, W., Li, H., Chen, C.-C.*. Structural insights to heterodimeric cis-prenyltransferases through yeast dehydrodolichyl diphosphate synthase subunit Nus1. Biochemical and Biophysical Research Communications, 2019, 515, 621-626.
40. Zhang, L., Ko, T.-P., Malwal S. R., Liu W., Zhou S., Oldfield E., Guo R. T.,* and Chen, C.-C.*. Complex structures of MoeN5 with substrate analogues suggest sequential catalytic mechanism, Biochemical and Biophysical Research Communications, 2019, 511, 800-805
41. Chang, Z., Ansbacher, T., Zhang, L., Yang, Y., Ko, T.-P., Zhang, G., Liu, W., Huang, J.-W., Dai, L., Guo, R.-T., Major, D. T.,* Chen, C.-C.*. Crystal structure of LepI, a multifunctional SAM-dependent enzyme which catalyzes pericyclic reactions in leporin biosynthesis, Organic Biomolecular Chemistry, 2019, 17, 2070-2076. (Cover story)
42. Malwal, S. R., Chen, L., Hicks, H., Qu, F., Liu, W., Shillo, A., Law, W. X., Zhang, J., Chandnani, N., Han, X., Zheng, Y., Chen, C.-C., Guo, R.-T., AbdelKhalek, A., Seleem, M. N., Oldfield, E. Discovery of lipophilic bisphosphonates that target bacterial cell wall and quinone biosynthesis. Journal of Medicinal Chemistry, 2019, 62, 2564-2581.
43. Zhang, M., Yu, X.-W., Xu, Y., Guo, R.-T., Swapna, G. V. T, Szyperski, T., Hunt, J. F., Montelione, G. T. Structural Basis by Which the N-Terminal Polypeptide Segment of Rhizopus chinensis Lipase Regulates Its Substrate Binding Affinity. Biochemistry, 2019, 58, 3943-3954.
44. Chen, C.-C.,# Hu, X.,# Tang, X.,# Yang, Y.,# Ko, T.-P., Gao, J., Zheng, Y., Huang, J.-W., Yu, Z., Li, L., Han, S., Cai, N., Zhang, Y.,* Liu, W.,* Guo, R.-T.*. The crystal structure of a class of cyclases that catalyze the Cope rearrangement. Angewandte Chemie International Edition, 2018, 57, 15060-15064. (Cover story)
45. Gao, J.,# Ko, T.-P.,# Chen, L.,# Malwal, S. R., Zhang, J., Hu, X., Qu, F., Liu, W., Huang, J.-W., Cheng, Y.-S., Chen, C.-C., Yang, Y., Zhang, Y., Oldfield, E., Guo, R.-T.*. “Head-to-middle” and “head-to-tail” cis-prenyl transferases: Structure of isosesquilavandulyl diphosphate synthase. Angewandte Chemie International Edition, 2018, 57, 683-687. (Cover story)
46. Chen, C.-C.,# Han, X.,# Ko, T.-P., Liu, W.,* Guo, R.-T.*. Structural studies reveal the molecular mechanism of PETase. FEBS Journal, 2018, 285, 3717-3723.
47. You, S., Chen, C.-C., Tu, T., Wang, X., Ma, R., Cai, H.-Y., Guo, R.-T.,* Luo, H.-Y.,* Yao, B.*. Insight into the functional roles of Glu175 in the hyperthermostable xylanase XYL10C-ΔN through structural analysis and site-saturation mutagenesis. Biotechnol Biofuels, 2018, 11, 159.
48. Zheng, Y.,# Liu, W.,# Chen C. C.,# Hu, X., Liu, W., Ko, T.-P., Tang, X., Wei, H., Huang, J.-W., Guo, R.-T.*. Crystal structure of a mycoestrogen-detoxifying lactonase from Rhinocladiella mackenziei: Molecular insight into ZHD substrate selectivity. ACS Catalysis. 2018, 8, 4294-4298.
49. Malwal, S. R.,# Gao, J.,# Hu, X.,# Yang, Y.,# Liu, W., Huang, J.-W., Ko, T.-P., Li, L., Chen, C.-C., O’Dowd, B., Khade, R. L., Zhang, Y., Zhang, Y., Oldfield, E., Guo, R.-T.*. Catalytic role of conserved asparagine, glutamine, serine, and tyrosine residues in isoprenoid biosynthesis enzymes ACS Catalysis. 2018, 8, 4299-4312.
50. Lee, S. M.-Y., Yip, T., Yan, S., Jin, D., Wei, H., Guo, R.-T., Peiris, JS Malik.*. Recognition of double-stranded RNAand regulation of interferon pathway by Toll-likereceptor10. Frontiers in Immunology. 2018, 9, 516.
51. Wang, J.,# Chen, C.-C.,# Yang, Y.,# Liu, W., Ko, T.-P., Shang, N., Hu, X., Xie, Y., Huang, J.-W., Zhang, Y.,* Guo, R.-T.*. Structural insight into a novel indole prenyltransferase in hapalindole-type alkaloid biosynthesis. Biochemical and Biophysical Research Communications, 2018, 495, 1782-1788.
52. Han, X.,# Liu, W.,# Huang, J.-W.,# Ma, J., Zheng, Y., Ko, T.-P., Xu, L., Cheng, Y.-S., Chen, C.-C., Guo, R.-T.*. Structural insight into catalytic mechanism of PET hydrolase. Nature Communications, 2017, 13, 2106.
53. Gao, J.,# Huang, J.-W.,# Li, Q.,# Liu, W., Ko, T.-P., Zheng, Y., Xiao, X., Kuo, C.-J., Chen, C.-C.,* Guo, R.-T.*. Characterization and crystal structure of a thermostable glycoside hydrolase family 45 1,4-β-endoglucanase from Thielavia terrestris. Enzyme and Microbial Technology, 2017, 99, 32-37.
54. Schwalen, C. J., Feng, X., Liu, W., O-Dowd, B., Ko, T.-P., Shin, C. J., Guo, R.-T., Mitchell, D. A.,* Oldfield, E.*. Head-to-head prenyl synthases in pathogenic bacteria. ChemBioChem : a European Journal of Chemical Biology, 2017, 18, 985-991.
55. Hui, R.,# Hu, X.,# Liu, W., Zheng, Y., Chen, Y., Guo, R.-T., Jin, J.,* Chen, C.-C.*. Characterization and crystal structure of a novel zearalenone hydrolase from Cladophialophora bantiana. Acta Crystallographica. Section F, Structural Biology Communications, 2017, 73, 515-519.
56. Zhang, L.,# Chen, C.-C.,# Ko, T.-P.,# Huang, J.-W., Zheng, Y., Liu, W., Wang, I., Malwal, S. R., Feng, X., Wang, K., Huang, C. C., Hsu, S.-T. D., Wang, A. H.-J., Oldfield, E.,* and Guo, R.-T.*. Moenomycin biosynthesis: structure and mechanism of action of the prenyl transferase MoeN5, Angewandte Chemie International Edition, 2016, 55, 4716-4720.
57. Xu, Z.,# Liu, W.,# Chen, C.-C.,# Li Q., Huang, J.-W., Ko, T.-P., Liu, G., Liu, W., Peng, W., Cheng, Y.-S., Chen, Y., Jin, J., Li, H.,* Zheng, Y.,* Guo, R.-T.*. Enhanced α‑zearalenol hydrolyzing activity of a mycoestrogen detoxifying lactonase by structure-based engineering. ACS Catalysis, 2016, 6, 7657-7663. (Cover story)
58. Liu, M.,# Chen, C.-C.,# Chen, Lu.,# Xiao, X., Zheng, Y.,Huang, J.-W., Liu, W., Ko, T.-P., Cheng, Y.-S., Feng, X., Oldfield, E.,* Guo, R.-T.,* and Ma, Y.*. Structure and function of a “head-to-middle” prenyltransferase: Lavandulyl diphosphate Synthase, Angewandte Chemie International Edition, 2016, 55, 4721-4724. (Cover story)
59. Wang, Y., Desai, J., Zhang, Y., Malwal, S. R., Shin, C. J., Feng, X., Sun, H., Liu, G., Guo, R.-T., Oldfield, E.*. Bacterial dell growth inhibitors targeting undecaprenyl diphosphate synthase and undecaprenyl diphosphate phosphatase. ChemMedChem, 2016, 11, 2311-2319.
60. Yan, J.,# Liu, W.,# Li, Y., Lai, H.-L., Zheng, Y., Huang, J.-W., Chen, C.-C., Chen, Y., Jin, J., Li, H.,* Guo, R.-T.*. Functional and structural analysis of Pichia pastoris expressed Aspergillus niger 1,4-β-endoglucanase. Biochemical and Biophysical Research Communications, 2016, 17, 8-12.
61. Desai, J., Liu, Y.-L., Wei, H., Liu, W., Ko, T.-P., Guo, R.-T., Oldfield, E.*.Structure, function, and inhibition of Staphylococcus aureus heptaprenyl diphosphate synthase. ChemMedChem, 2016, 11, 1915-1923.
62. Huang, J.-W.,# Liu, W.,# Lai, H.-L., Cheng, Y.-S., Zheng, Y., Li, Q., Sun, H., Kuo, C.-J., Guo, R.-T.,* Chen, C.-C.*. Crystal structure and genetic modifications of FI-CMCase from Aspergillus aculeatus F-50. Biochemical and Biophysical Research Communications, 2016, 16, 565-572.
63. Yang, Y.,# Ko, T.-P.,# Chen, C.-C.,# Huang, G., Zheng, Y., Liu, W., Wang, I., Ho, M., Hsu, S., O'Dowd, B., Huff, H., Huang, C.-H., Docampo, R., Oldfield, E.,* Guo, R.-T.*. Structures of trypanosome vacuolar soluble pyrophosphatases: Anti-parasitic drug targets. ACS Chemical Biology, 2016, 11, 1362-1371.
64. Zheng, Y.,# Li, Y.,# Liu, W.,# Chen, C.-C., Ko, T.-P., He, M., Xu, Z., Liu, M., Luo, H., Guo, R.-T.*. Yao, B,* and Ma, Y.* Structural insight into potential cold adaptation mechanism through a psychrophilic glycoside hydrolase family 10 endo-β-1, 4-xylanase. Journal of Structural Biology, 2016, 193, 206-211. (Cover story)
65. Liu, G., Li, Q., Shang, N., Huang, J.-W., Ko, T.-P., Liu, W., Zheng, Y., Han, X., Chen, Y., Chen, C.-C., Jin, J., Guo, R.-T.*. Functional and structural analyses of a 1,4- β-endoglucanase from Ganoderma lucidum. Enzyme and Microbial Technology, 2016, 86, 67-74.
66. Hu, Y.,# Liu, W.,# Malwal, S. R.,Zheng, Y., Feng, X., Ko, T.-P., Chen, C.-C., Xu, Z., Liu, M., Han, X., Gao, J., Oldfield, E.,* Guo, R.-T.*. Structures of iridoid synthase from Cantharanthus roseus with bound NAD(+), NADPH, or NAD(+) /10-oxogeranial: Reaction mechanisms. Angewandte Chemie International Edition, 2015, 54, 15478-15482. (Cover story)
67. Cheng, Y.-S., Chen, C.-C., Huang, J.-W., Ko, T.-P., Huang, Z., Guo, R.-T.*. Improving the catalytic performance of a GH11 xylanase by rational protein engineering. Applied Microbiology and Biotechnology, 2015, 99, 9503-9510.
68. Zheng, Y., Chen, C.-C., Ko, T.-P., Xiao, X., Yang, Y., Huang, C.-H., Qian, G., Shao, W.,* Guo, R.-T.*. Crystal structures of S-adenosyl homocysteine hydrolase from the thermophilic bacterium Thermotoga maritima. Journal of Structural Biology, 2015, 190, 135-142. (Cover story)
69. Guo, R.-T.*. Green Enzymes. ChemBioEng Reviews, 2015, 2, 75. (Invited Editorial)
70. Chen, C.-C., Cheng, K. J., Ko, T.-P., Guo, R.-T.*. Current progresses in phytase research: Three-dimensional structure and protein engineering. ChemBioEng Reviews, 2015, 2, 76-86. (Cover story)
71. Chen, C.-C., Ko, T.-P., Huang, J.-W., Guo, R.-T.*. Heat- and alkaline-stable xylanases: applications, protein structure and engineering. ChemBioEng Reviews, 2015, 2, 95-106.
72. Chan, H.-C.,# Ko, T.-P.,# Huang, C.-H., Guo, R.-T.*. Minireview: A comeback of Hg-derivatives in protein crystallography with Cys-modification. ChemBioEng Reviews, 2015, 2, 133-140.
73. Liu, W., Guo, R.-T., Chen, X., Li, Z., Gao, X., Huang, C.-H., Wu, Q., Feng, J., Zhu, D.*. Structural analysis reveals the substrate-binding mechanism for the expanded substrate specificity of mutant meso-diaminopimelate dehydrogenase. ChemBioChem : a European Journal of Chemical Biology, 2015, 16, 924-929. (Cover story)
74. Chen, Y., Huang, J.-W., Chen, C.-C., Lai, H.-L., Jin, J., Guo, R.-T.*. Crystallization and preliminary X-ray diffraction analysis of an endo-1, 4-beta-D-glucanase from Aspergillus aculeatus F-50. Acta Crystallographica. Section F, Structural Biology Communications, 2015, 71, 397-400.
75. Zhu, W., Wang, Y., Li, K., Gao, J., Huang, C.-H., Chen, C.-C., Ko, T.-P., Zhang, Y., Guo, R.-T., Oldfield, E.*. Antibacterial drug leads: DNA and enzyme multi-targeting. Journal of Medicinal Chemistry, 2015, 58, 1215-1227.
76. Zheng, Y., # Ko, T.-P., # Yang, Y., Shao, W., * Guo, R.-T.*. Binding mode of the oxidized alpha-anomer of NAD+ to RSP, a Rex-family repressor. Biochemical and Biophysical Research Communications, 2015, 456, 733-736.
77. Yuan, S.-F., Wu, T.-H., Lee, H.-L., Hsieh, H.-Y., Lin, W.-L., Yang, B., Chang, C.-K., Li, Q., Gao, J., Huang, C.-H., Ho, M.-C.,* Guo, R.-T.*. Liang, P.-H.* Biochemical characterization and structural analysis of a bi-functional cellulase/xylanase from Clostridium thermocellum. The Journal of Biological Chemistry, 2015, 290, 5739-5748.
78. Chen, C.-C., Huang, J.-W., Zhao, P., Ko, T.-P., Huang, C.-H., Chan, H.-C., Huang, Z., Liu, W., Cheng, Y.-S., Liu, J.-R., Guo, R.-T.*. Structural analyses and yeast production of the β-1,3-1,4-glucanase catalytic module encoded by the licB gene of Clostridium thermocellum. Enzyme and Microbial Technology, 2015, 71, 1-7.
79. Veiga-Santos, P., Li, K., Lameira, L., de Carvalho, T. M., Huang, G., Galizzi, M., Shang, N., Li, Q., Gonzalez-Pacanowska, D., Hernandez-Rodriguez, V., Benaim, G., Guo, R.-T., Urbina, J. A., Docampo, R., de Souza, W., Oldfield, E.*. SQ109: A new drug lead for Chagas disease. Antimicrobial Agents and Chemotherapy, 2015, 59, 1950-1961.
80. Han, X., # Chen, C.-C., # Kuo, C.-J., Huang, C.-H., Zheng, Y., Ko, T.-P., Zhu, Z., Feng, X., Wang, K., Oldfield, E., Wang, A. H.-J., Liang, P.-H., Guo, R.-T.,* Ma, Y.*. Crystal structures of ligand-bound octaprenyl pyrophosphate synthase from Escherichia coli reveal the catalytic and chain-length determining mechanisms. Proteins, 2015, 83, 37-45.
81. Chan, H.-C.,# Feng, X.,# Ko, T.-P.#, Huang, C.-H., Hu, Y., Zheng, Y., Bogue, S., Nakano, C., Hoshino, T., Zhang, L., Lv, P., Liu, W., Crick, D. C., Liang, P.-H., Wang, A. H.-J., Oldfield, E.,* Guo, R.-T.*. Structure and inhibition of tuberculosinol synthase and decaprenyl diphosphate synthase from Mycobacterium tuberculosis. Journal of the American Chemical Society, 2014, 136, 2892-2896.
82. Liu, W., # Feng, X., # Zheng, Y., # Huang, C.-H., Nakano, C., Hoshino, T., Bogue, S., Ko, T.-P., Chen, C.-C., Cui, Y., Li, J., Wang, I., Hsu, S.-T. D., Oldfield, E.,* Guo, R.-T.*. Structure, function and inhibition of ent-kaurene synthase from Bradyrhizobium japonicum. Scientific Reports, 2014, 4, 6214.
83. Cheng, Y.-S., # Chen, C.-C., # Huang, C.-H., Ko, T.-P., Luo, W., Huang, J.-W., Liu, J.-R., Guo, R.-T.*. Structural analysis of a glycoside hydrolase family 11 xylanase from Neocallimastix patriciarum: insights into the molecular basis of a thermophilic enzyme. The Journal of Biological Chemistry, 2014, 289, 11020-11028.
84. Shang, N., # Li, Q., # Ko, T.-P., # Chan, H.-C., Li, J., Zheng, Y., Huang, C.-H., Ren, F., Chen, C.-C., Zhu, Z., Galizzi, M., Li, Z. H., Rodrigues-Poveda, C. A., Gonzalez-Pacanowska, D., Veiga-Santos, P., de Carvalho, T. M., de Souza, W., Urbina, J. A., Wang, A. H.-J., Docampo, R., Li, K., Liu, Y.-L., Oldfield, E.,* Guo, R.-T.*. Squalene synthase as a target for Chagas disease therapeutics. PLoS Pathogens, 2014, 10, e1004114.
85. Zheng, Y., # Ko, T.-P., # Sun, H., Huang, C.-H., Pei, J., Qiu, R., Wang, A. H.-J., Wiegel, J., Shao, W.,* Guo, R.-T.*. Distinct structural features of Rex-family repressors to sense redox levels in anaerobes and aerobes. Journal of Structural Biology, 2014, 188, 195-204. (Cover story)
86. Peng, W., # Ko, T.-P., # Yang, Y., Zheng, Y., Chen, C.-C., Zhu, Z., Huang, C.-H., Zeng, Y.-F., Huang, J.-W., Wang, A. H.-J.-J., Liu, J.-R.,* Guo, R.-T.*. Crystal structure and substrate-binding mode of the mycoestrogen-detoxifying lactonase ZHD from Clonostachys rosea. RSC Advances, 2014, 4, 62321-62325.
87. Zhu, Z., # He, M., # Huang, C.-H., Ko, T.-P., Zeng, Y.-F., Huang, Y.-N., Jia, S., Lu, F., Liu, J.-R., Guo, R.-T.*. Crystallization and preliminary X-ray diffraction analysis of a novel beta-L-arabinofuranosidase (HypBA1) from Bifidobacterium longum. Acta Crystallographica. Section F, Structural Biology Communications, 2014, 70, 636-638.
88. Huang, C.-H., Zhu, Z., Cheng, Y.-S., Chan, H.-C., Ko, T.-P., Chen, C.-C., Wang, I., Ho, M.-R., Hsu, S.-T. D. -D., Zeng, Y.-F., Huang, Y.-N., Liu, J.-R.,* Guo, R.-T.*. Structure and Catalytic Mechanism of a Glycoside Hydrolase Family-127β-L-Arabinofuranosidase (HypBA1). J Bioprocess Biotech, 2014, 4, 171.
89. Zhang, L., # Zhao, P., # Chen, C.-C., Huang, C.-H., Ko, T.-P., Zheng, Y., Guo, R.-T.*. Preliminary X-ray diffraction analysis of a thermophilic beta-1,3-1,4-glucanase from Clostridium thermocellum. Acta Crystallographica. Section F, Structural Biology Communications, 2014, 70, 946-948.
90. Yang, Y., # Ko, T.-P., # Liu, L., Li, J., Huang, C.-H., Chen, J., Guo, R.-T.,* Du, G.*. Roles of tryptophan residue and disulfide bond in the variable lid region of oxidized polyvinyl alcohol hydrolase. Biochemical and Biophysical Research Communications, 2014, 452, 509-514.
91. Yang, Y., # Ko, T.-P., # Liu, L., Li, J., Huang, C.-H., Chan, H.-C., Ren, F., Jia, D., Wang, A. H.-J., Guo, R.-T.,* Chen, J.,* Du, G.*. Structural insights into enzymatic degradation of oxidized polyvinyl alcohol. ChemBioChem : a European Journal of Chemical Biology, 2014, 15, 1882-1886. (Cover story)
92. Xu, J., # Ren, F., # Huang, C.-H., Zheng, Y., Zhen, J., Sun, H., Ko, T.-P., He, M., Chen, C.-C., Chan, H.-C., Guo, R.-T.,* Song, H.,* Ma, Y.*. Functional and structural studies of pullulanase from Anoxybacillus sp. LM18-11. Proteins, 2014, 82, 1685-1693. (Cover story)
93. Wu, T.-H., # Chen, C.-C., # Cheng, Y.-S., Ko, T.-P., Lin, C.-Y., Lai, H.-L., Huang, T.-Y., Liu, J.-R., * Guo, R.-T.*. Improving specific activity and thermostability of Escherichia coli phytase by structure-based rational design. Journal of Biotechnology, 2014, 175, 1-6.
94. Wang, S., Nie, Y., Xu, Y.,* Ko, T.-P., Huang, C.-H., Chan, H.-C., Guo, R.-T.,* Xiao, R. Crystallization and preliminary X-ray diffraction analysis of (R)-carbonyl reductase from Candida parapsilosis. Acta Crystallographica. Section F, Structural Biology Communications, 2014, 70, 800-802.
95. Wang, S., Nie, Y., Xu, Y.,* Zhang, R., Ko, T.-P., Huang, C.-H., Chan, H.-C., Guo, R.-T.,* Xiao, R. Unconserved substrate-binding sites direct the stereoselectivity of medium-chain alcohol dehydrogenase. Chemical Communications, 2014, 50, 7770-7772. (Cover story)
96. Tseng, C.-W., # Liao, C.-Y., # Sun, Y., Peng, C.-C., Tzen, J. T., Guo, R.-T.,* Liu, J.-R.*. Immobilization of Clostridium cellulolyticum D-psicose 3-epimerase on artificial oil bodies. Journal of Agricultural and Food Chemistry, 2014, 62, 6771-6776.
97. Lv, P., # Zhang, L., # Luo, H., Chen, C.-C., Huang, C.-H., Peng, W., Wang, K., Ko, T.-P., Zheng, Y., Zhang, J., Yao, B.,* Guo, R.-T.*. Preliminary X-ray diffraction analysis of thermostable beta-1,4-xylanase from Streptomyces sp. S9. Acta Crystallographica. Section F, Structural Biology Communications, 2014, 70, 105-107.
98. Liu, W., Li, Z., Huang, C.-H., Guo, R.-T., Zhao, L., Zhang, D., Chen, X., Wu, Q., Zhu, D.*. Structural and mutational studies on the unusual substrate specificity of meso-diaminopimelate dehydrogenase from Symbiobacterium thermophilum. ChemBioChem : a European Journal of Chemical Biology, 2014, 15, 217-222. (Cover story)
99. Li, K., Schurig-Briccio, L. A., Feng, X., Upadhyay, A., Pujari, V., Lechartier, B., Fontes, F. L., Yang, H., Rao, G., Zhu, W., Gulati, A., No, J.-H., Cintra, G., Bogue, S., Liu, Y.-L., Molohon, K., Orlean, P., Mitchell, D. A., Freitas-Junior, L., Ren, F., Sun, H., Jiang, T., Li, Y., Guo, R.-T., Cole, S. T., Gennis, R. B., Crick, D. C., Oldfield, E.*. Multitarget drug discovery for tuberculosis and other infectious diseases. Journal of Medicinal Chemistry, 2014, 57, 3126-3139.
100. Huang, J.-W., # Chen, C.-C., # Huang, C.-H., Huang, T.-Y., Wu, T.-H., Cheng, Y.-S., Ko, T.-P., Lin, C.-Y., Liu, J.-R.,* Guo, R.-T.*. Improving the specific activity of beta-mannanase from Aspergillus niger BK01 by structure-based rational design. Biochimica et Biophysica Acta, 2014, 1844, 663-669. (Cover story)
101. He, M., # Zheng, Y., # Huang, C.-H., Qian, G., Xiao, X., Ko, T.-P., Shao, W., Guo, R.-T.*. Crystallization and preliminary X-ray diffraction analysis of the S-adenosylhomocysteine hydrolase (SAHH) from Thermotoga maritima. Acta Crystallographica. Section F, Structural Biology Communications, 2014, 70, 1563-1565.
102. Feng, X., # Hu, Y., # Zheng, Y., # Zhu, W., Li, K., Huang, C.-H., Ko, T.-P., Ren, F., Chan, H.-C., Nega, M., Bogue, S., Lopez, D., Kolter, R., Gotz, F., Guo, R.-T.,* Oldfield, E.*. Structural and functional analysis of Bacillus subtilis YisP reveals a role of its product in biofilm production. Chemistry & Biology, 2014, 21, 1557-1563.
103. Cheng, Y.-S., # Huang, C.-H., # Chen, C.-C., Huang, T.-Y., Ko, T.-P., Huang, J.-W., Wu, T.-H., Liu, J.-R.,* Guo, R.-T.*. Structural and mutagenetic analyses of a 1,3-1,4-beta-glucanase from Paecilomyces thermophila. Biochimica et Biophysica Acta, 2014, 1844, 366-373. (Cover story)
104. Chen, C.-C., # Luo, H., # Han, X., # Lv, P., Ko, T.-P., Peng, W., Huang, C.-H., Wang, K., Gao, J., Zheng, Y., Yang, Y., Zhang, J., Yao, B.,* Guo, R.-T.*. Structural perspectives of an engineered beta-1,4-xylanase with enhanced thermostability. Journal of Biotechnology, 2014, 189, 175-182.
105. Bai, W., Zhou, C., Xue, Y., Huang, C.-H., Guo, R.-T., Ma, Y.*. Three-dimensional structure of an alkaline xylanase Xyn11A-LC from alkalophilic Bacillus sp. SN5 and improvement of its thermal performance by introducing arginines substitutions. Biotechnology Letters, 2014, 36, 1495-1501.
106. Ren, F., # Feng, X., # Ko, T.-P., # Huang, C.-H., Hu, Y., Chan, H.-C., Liu, Y.-L., Wang, K., Chen, C.-C., Pang, X., He, M., Li, Y., Oldfield, E.,* Guo, R.-T.*. Insights into TIM-barrel prenyl transferase mechanisms: crystal structures of PcrB from Bacillus subtilis and Staphylococcus aureus. ChemBioChem : a European Journal of Chemical Biology, 2013, 14, 195-199. (Cover story)
107. Luo, W., # Huang, J.-W., # Huang, C.-H., Huang, T.-Y., Chan, H.-C., Liu, J.-R., Guo, R.-T., Chen, C.-C.*. Preliminary X-ray diffraction analysis of thermostable beta-1,4-mannanase from Aspergillus niger BK01. Acta Crystallographica. Section F, Structural Biology and Crystallization Communications, 2013, 69, 1100-1102.
108. Li, X., # Han, X., # Ko, T.-P., Chen, C.-C., Zhu, Z., Hua, E., Guo, R.-T., Huang, C.-H.*. Preliminary X-ray diffraction analysis of octaprenyl pyrophosphate synthase from Escherichia coli. Acta crystallographica. Section F, Structural Biology and Crystallization Communications, 2013, 69, 328-331.
109. Jiang, T., # Chan, H.-C., # Huang, C.-H., Ko, T.-P., Huang, T.-Y., Liu, J.-R., Guo, R.-T.*. Substrate binding to a GH131 beta-glucanase catalytic domain from Podospora anserina. Biochemical and Biophysical Research Communications, 2013, 438, 193-197.
110. Hu, Y., Jia, S., Ren, F., Huang, C.-H., Ko, T.-P., Mitchell, D. A., Guo, R.-T., Zheng, Y.*. Crystallization and preliminary X-ray diffraction analysis of YisP protein from Bacillus subtilis subsp. subtilis strain 168. Acta Crystallographica. Section F, Structural Biology and Crystallization Communications, 2013, 69, 77-79.
111. Han, X., # Gao, J., # Shang, N., # Huang, C.-H., Ko, T.-P., Chen, C.-C., Chan, H.-C., Cheng, Y.-S., Zhu, Z., Wiegel, J., Luo, W.,*Guo, R.-T.,* Ma, Y.*. Structural and functional analyses of catalytic domain of GH10 xylanase from Thermoanaerobacterium saccharolyticum JW/SL-YS485. Proteins, 2013, 81, 1256-1265. (Cover story)
112. Chang, T.-H., # Chang, S. J., # Hsieh, F. L., Ko, T.-P., Lin, C.-T., Ho, M.-R., Wang, I., Hsu, S.-T. D., Guo, R.-T., Chang, W., Wang, A. H.-J.*. Crystal structure of vaccinia viral A27 protein reveals a novel structure critical for its function and complex formation with A26 protein. PLoS Pathogens, 2013, 9, e1003563.
113. Ren, F.,# Ko, T.-P.,# Feng, X., Huang, C.-H., Chan, H.-C., Hu, Y., Wang, K., Ma, Y., Liang, P.-H., Wang, A. H.-J., Oldfield, E.,* Guo, R.-T.*. Insights into the mechanism of the antibiotic-synthesizing enzyme MoeO5 from crystal structures of different complexes. Angewandte Chemie International Edition, 2012, 51, 4157-4160. (Cover story)
114. Zheng, Y., Huang, C.-H., Liu, W., Ko, T.-P., Xue, Y., Zhou, C., Guo, R.-T., Ma, Y.*. Crystal structure and substrate-binding mode of a novel pectate lyase from alkaliphilic Bacillus sp. N16-5. Biochemical and Biophysical Research Communications, 2012, 420, 269-274.
115. Liu, W., Sun, Y., Ko, T.-P., Wiegel, J., Shao, W., Lu, F., Guo, R.-T., Huang, C.-H.*. Crystallization and preliminary X-ray diffraction analysis of a novel GH120 beta-xylosidase (XylC) from Thermoanaerobacterium saccharolyticum JW/SL-YS485. Acta Crystallographica. Section F, Structural Biology and Crystallization Communications, 2012, 68, 914-916.
116. Huang, J.-W., Cheng, Y.-S., Ko, T.-P., Lin, C.-Y., Lai, H.-L., Chen, C.-C., Ma, Y., Zheng, Y., Huang, C.-H., Zou, P., Liu, J.-R.,* Guo, R.-T.*. Rational design to improve thermostability and specific activity of the truncated Fibrobacter succinogenes 1,3-1,4-beta-D-glucanase. Applied Microbiology and Biotechnology, 2012, 94, 111-121.
117. Huang, C.-H.,# Sun, Y.,# Ko, T.-P., Chen, C.-C., Zheng, Y., Chan, H.-C., Pang, X., Wiegel, J.,* Shao, W.,* Guo, R.-T.*. The substrate/product-binding modes of a novel GH120 beta-xylosidase (XylC) from Thermoanaerobacterium saccharolyticum JW/SL-YS485. The Biochemical Journal, 2012, 448, 401-407.
118. Cheng, Y.-S., Ko, T.-P., Huang, J.-W., Wu, T.-H., Lin, C.-Y., Luo, W., Li, Q., Ma, Y., Huang, C.-H., Wang, A. H.-J., Liu, J.-R.,* Guo, R.-T.*. Enhanced activity of Thermotoga maritima cellulase 12A by mutating a unique surface loop. Applied Microbiology and Biotechnology, 2012, 95, 661-669.
119. Chang, K.-M., Chen, S.-H., Kuo, C.-J., Chang, C.-K., Guo, R.-T., Yang, J. M., Liang, P.-H.*. Roles of amino acids in the Escherichia coli octaprenyl diphosphate synthase active site probed by structure-guided site-directed mutagenesis. Biochemistry, 2012, 51, 3412-3419.
120. Chan, H.-C.,# Zhu, Y.,# Hu, Y., Ko, T.-P., Huang, C.-H., Ren, F., Chen, C.-C., Ma, Y., Guo, R.-T.,* Sun, Y.*. Crystal structures of D-psicose 3-epimerase from Clostridium cellulolyticum H10 and its complex with ketohexose sugars. Protein & Cell, 2012, 3, 123-131. (Cover story)
121. Pai, C. K. Wang, H. T., Guo, R.-T., Liu, J.-R.*. The construction of bifunctional fusion xylanolytic enzymes and the prediction of optimum reaction conditions for the enzyme activity. BioResources, 2012, 7, 5647-5665.
122. Zeng, Y.-F., # Ko, T.-P., # Lai, H.-L., Cheng, Y.-S., Wu, T.-H., Ma, Y., Chen, C.-C., Yang, C. S., Cheng, K. J., Huang, C.-H., Guo, R.-T.,* Liu, J.-R.*. Crystal structures of Bacillus alkaline phytase in complex with divalent metal ions and inositol hexasulfate. Journal of Molecular Biology, 2011, 409, 214-224. (Cover story)
123. Wu, T.-H.,# Huang, C.-H.,# Ko, T.-P., Lai, H.-L., Ma, Y., Chen, C.-C., Cheng, Y.-S., Liu, J.-R.,* Guo, R.-T.*. Diverse substrate recognition mechanism revealed by Thermotoga maritima Cel5A structures in complex with cellotetraose, cellobiose and mannotriose. Biochimica et Biophysica Acta, 2011, 1814, 1832-1840. (Cover story)
124. Tseng, C.-W.,# Ko, T.-P.,# Guo, R.-T.,# Huang, J.-W., Wang, H.-C., Huang, C.-H., Cheng, Y.-S., Wang, A. H.-J., Liu, J.-R.*. Substrate binding of a GH5 endoglucanase from the ruminal fungus Piromyces rhizinflata. Acta Crystallographica. Section F, Structural Biology and Crystallization Communications, 2011, 67, 1189-1194. (Cover story)
125. Cheng, Y.-S., Ko, T.-P., Wu, T.-H., Ma, Y., Huang, C.-H., Lai, H.-L., Wang, A. H.-J., Liu, J.-R.,* Guo, R.-T.*. Crystal structure and substrate-binding mode of cellulase 12A from Thermotoga maritima. Proteins, 2011, 79, 1193-1204. (Cover story)
126. Zhang, Y., Cao, R., Yin, F., Hudock, M. P., Guo, R.-T., Krysiak, K., Mukherjee, S., Gao, Y.-G., Robinson, H., Song, Y., No, J.-H., Bergan, K., Leon, A., Cass, L., Goddard, A., Chang, T.-K., Lin, F.-Y., Van Beek, E., Papapoulos, S., Wang, A. H.-J., Kubo, T., Ochi, M., Mukkamala, D., Oldfield, E.*. Lipophilic bisphosphonates as dual farnesyl/geranylgeranyl diphosphate synthase inhibitors: an X-ray and NMR investigation. Journal of the American Chemical Society, 2009, 131, 5153-5162.
127. Guo, R.-T.,# Chong, Y.-E.,# Guo, M., Yang, X.-L.*. Crystal structures and biochemical analyses suggest a unique mechanism and role for human glycyl-tRNA synthetase in Ap4A homeostasis. The Journal of Biological Chemistry, 2009, 284, 28968-28976.
128. Chen, C.-K., Lee, G.-C., Ko, T.-P., Guo, R.-T., Huang, L.-M., Liu, H.-J., Ho, Y.-F., Shaw, J.-F., Wang, A. H.-J.*. Structure of the alkalohyper thermophilic Archaeoglobus fulgidus lipase contains a unique C-terminal domain essential for long-chain substrate binding. Journal of Molecular Biology, 2009, 390, 672-685.
129. Wu, Y.-H., Ko, T.-P., Guo, R.-T., Hu, S.-M., Chuang, L.-M., Wang, A. H.-J.*. Structural basis for catalytic and inhibitory mechanisms of human prostaglandin reductase PTGR2. Structure, 2008, 16, 1714-1723.
130. Kuo, C.-J., Guo, R.-T., Lu, I.-L., Liu, H.-G., Wu, S.-Y., Ko, T.-P., Wang, A. H.-J., Liang, P.-H.*. Structure-based inhibitors exhibit differential activities against Helicobacter pylori and Escherichia coli undecaprenyl pyrophosphate synthases. Journal of Biomedicine & Biotechnology, 2008, 841312.
131. Jeng, W.-Y., Ko, T.-P., Liu, C.-I., Guo, R.-T., Liu, C.-L., Shr, H.-L., Wang, A. H.-J.*. Crystal structure of IcaR, a repressor of the TetR family implicated in biofilm formation in Staphylococcus epidermidis. Nucleic Acids Research, 2008, 36, 1567-1577.
132. Cao, R., Chen, C.-K. C., Guo, R.-T., Wang, A. H.-J.,* Oldfield, E.*. Structures of a potent phenylalkyl bisphosphonate inhibitor bound to farnesyl and geranylgeranyl diphosphate synthases. Proteins, 2008, 73, 431-439.
133. Chen, K.-M. C., Hudock, M. P., Zhang, Y., Guo, R.-T., Cao, R., No, J.-H., Liang, P.-H., Ko, T.-P., Chang, T.-H., Chang, S.-C., Song, Y., Axelson, J., Kumar, A., Wang, A. H.-J., Oldfield, E.*. Inhibition of geranylgeranyl diphosphate synthase by bisphosphonates: a crystallographic and computational investigation. Journal of Medicinal Chemistry, 2008, 51, 5594-5607.
134. Guo, R.-T.,# Cao, R.,# Liang, P.-H., Ko, T.-P., Chang, T.-H., Hudock, M. P., Jeng, W.-Y., Chen, C.-K., Zhang, Y., Song, Y., Kuo, C.-J., Yin, F., Oldfield, E., Wang, A. H.-J.*. Bisphosphonates target multiple sites in both cis- and trans-prenyltransferases. Proceedings of the National Academy of Sciences of the United States of America, 2007, 104, 10022-10027.
135. Chang, T.-H., Guo, R.-T., Ko, T.-P., Wang, A. H.-J.,* Liang, P.-H.*. Crystal structure of type-III geranylgeranyl pyrophosphate synthase from Saccharomyces cerevisiae and the mechanism of product chain length determination. The Journal of Biological Chemistry, 2006, 281, 14991-15000.
136. Sun, H.-Y., Ko, T.-P., Kuo, C.-J., Guo, R.-T., Chou, C.-C., Liang, P.-H.,* Wang, A. H.-J.*. Homodimeric hexaprenyl pyrophosphate synthase from the thermoacidophilic crenarchaeon Sulfolobus solfataricus displays asymmetric subunit structures. Journal of Bacteriology, 2005, 187, 8137-8148.
137. Guo, R.-T.,# Ko, T.-P.,# Chen, A. P., Kuo, C.-J., Wang, A. H.-J.,* Liang, P.-H.*. Crystal structures of undecaprenyl pyrophosphate synthase in complex with magnesium, isopentenyl pyrophosphate, and farnesyl thiopyrophosphate: roles of the metal ion and conserved residues in catalysis. The Journal of Biological Chemistry, 2005, 280, 20762-20774.
138. Guo, R.-T.,# Kuo, C.-J.,# Ko, T.-P., Chou, C.-C., Liang, P.-H.,* Wang, A. H.-J.*. A molecular ruler for chain elongation catalyzed by octaprenyl pyrophosphate synthase and its structure-based engineering to produce unprecedented long chain trans-prenyl products. Biochemistry, 2004, 43, 7678-7686.
139. Guo, R.-T., Kuo, C.-J., Chou, C.-C., Ko, T.-P., Shr, H.-L., Liang, P.-H.,* Wang, A. H.-J.*. Crystal structure of octaprenyl pyrophosphate synthase from hyperthermophilic Thermotoga maritima and mechanism of product chain length determination. The Journal of Biological Chemistry, 2004, 279, 4903-4912.
140. Chu, H.-M.,#Guo, R.-T.,# Lin, T. W., Chou, C.-C., Shr, H.-L., Lai, H.-L., Tang, T.-Y., Cheng, K.-J., Selinger, B. L., Wang, A. H.-J.*. Structures of Selenomonas ruminantium phytase in complex with persulfated phytate: DSP phytase fold and mechanism for sequential substrate hydrolysis. Structure, 2004, 12, 2015-2024.
141. Guo, R.-T., # Ko, T.-P., # Chou, C.-C., Shr, H.-L., Chu, H.-M., Tsai, Y.-H., Liang, P.-H., Wang, A. H.-J.*. Preliminary X-ray diffraction analysis of octaprenyl pyrophosphate synthase crystals from Thermotoga maritima and Escherichia coli. Acta Crystallographica. Section D, Biological Crystallography, 2003, 59, 2265-2268.
142. Guo, R.-T., Chou, L.-J., Chen, Y.-C., Chen, C.-Y., Pari, K., Jen, C. J., Lo, S. J., Huang, S.-L., Lee, C.-Y., Chang, T.-W., Chuang, W.-J.*. Expression in Pichia pastoris and characterization by circular dichroism and NMR of rhodostomin. Proteins, 2001, 43, 499-508.
六、已授权专利
1. 郭瑞庭，郑雅珊，吴姿慧，黄建文，赖惠琳，林正言。一种纤维素酶及其应用，授权号：ZL2011100426000，中国专利。
2. 郭瑞庭，黄建文，郑雅珊，吴姿慧，赖惠琳，林正言。酶活性及热稳定性被提高的葡聚糖酶，授权号：ZL2011100596815，中国专利。
3. 郭瑞庭，黄建文，郑雅珊，吴姿慧，赖惠琳，林正言，黄婷沅。提升酶活性之木聚糖酶，ZL2013101344011，中国专利。
4. 郭瑞庭，黄建文，郑雅珊，吴姿慧，赖惠琳，林正言，黄婷沅。酶活性提高的甘露聚糖酶，授权号：ZL201310135208X，中国专利。
5. 郭瑞庭，陈纯琪，吴姿慧，郑雅珊，黄建文，赖惠琳，林正言，黄婷沅。具有提升的酶活性的植酸酶，授权号：CN104342418B，中国专利。
6. 郭瑞庭，陈纯琪，吴姿慧，郑雅珊，黄建文，赖惠琳，林正言，黄婷沅。具有提升的酶活性的植酸酶，授权号：CN106399273B，中国专利。
7. Guo, R.-T., Zheng, Y., Xu, Z. , Chen, C.-C., Peng, W., Liu, W., Liu, J.-R., Ma, Y. Lactone hydrolase and method of degrading alpha-zearalenol using the same，授权号：US10316303B2，美国专利。
8. Guo, R.-T., Cheng, Y.-S., Wu, T.-H., Huang, J.-W., Lai, H.-L., Lin, C.-Y.，Thermostable cellulase having increased enzyme activity，授权号US8137945B1，美国专利。
9. Guo, R.-T., Huang, J.-W., Cheng, Y.-S., Wu, T.-H., Lai, H.-L., Lin C.-Y.，Glucanase having increased enzyme activity and thermostability，授权号US8192974B1，美国专利。
10. Guo, R.-T., Cheng, Y.-S., Huang, J.-W., Wu, T.-H., Lai, H.-L., Lin, C.-Y., Huang, T.-Y.，Xylanase having improved enzymatic activity，授权号US8722382B1，美国专利。
11. Guo, R.-T., Huang, J.-W., Cheng, Y.-S., Wu, T.-H., Lai, H.-L., Lin, C.-Y., Huang, T.-Y.，Beta-mannanase having improved enzymatic activity，授权号US8865444B1，美国专利。
12. Guo, R.-T., Huang, J.-W., Cheng, Y.-S., Wu, T.-H., Lai, H.-L., Lin C.-Y., Huang T.-Y. β-mannanase having improved production yield and enzymatic activity，授权号US8999695B1，美国专利。
13. Guo, R.-T., Wu, T.-H., Cheng, Y.-S., Huang, J.-W., Lai, H.-L., Lin C.-Y., Huang T.-Y. Phytase having improved enzymatic activity，授权号US9194010B2，美国专利。
14. Chen, C.-C., Huang, J.-W., Dai, L., Yu, X., Chen, C.-Y., Wu, S., Zhan, Z., Zhang, L., Zhai, C., Ma, L., Guo, R.-T. Cellulase having improved enzymatic activity，授权号：US10479985B1，美国专利。
15. Guo, R.-T., Chen, C.-C., Cheng, Y.-S., Huang, J.-W., Wu, T.-H., Lai, H.-L., Lin, C.-Y., Ko, T.-Y. Cellulase having improved enzymatic activity，授权号：US9809807B1，美国专利。
16. Wu, T.-H., Cheng, Y.-S., Lai, H.-L., Ke, T.-Y., Huang, J.-W., Chen, C.-C., Guo, R.-T. Phytase having improved thermostability，授权号：US10011822B2，美国专利。
17. Cheng, Y.-S., Wu, T.-H., Lin, C.-Y., Lai, H.-L., Zheng, C.-B., Huang, T.-Y., Lin, I.-H., Huang, J.-W., Chen, C.-C., Guo, R.-T. Glucose oxidase having improved thermostability，授权号：US10233430B1，美国专利。
18. Chen, C.-C., Wang, T., Huang, J.-W., Guo, R.-T. Lysozyme having improved enzymatic activity，授权号：US10323237B2，美国专利。
19. Cheng, Y.-S., Wu, T.-H., Lin, C.-Y., Lai, H.-L., Zheng, C.-B., Huang, T.-Y., Lin, I.-H., Huang, J.-W., Chen, C.-C., Guo, R.-T. Xylosidase having improved enzymatic activity，授权号：US10385346B2，美国专利。
七、获奖与荣誉称号
1. 2020年，“湖北省创新群体”(群体负责人)
2. 2019年，“湖北省省级人才入选者”
3. 2019年，“天津市自然科学二等奖”(第一完成人)
4. 2018年，“国家级人才入选者”
5. 2017年，“部级人才入选者”
6. 2015年，“天津市131第一层次人才培养工程”
7. 2012年，“天津市131第二层次人才培养工程”
8. 2012年，“2011年科学中国人年度人物”
9. 2010年，“中国科学院人才计划入选者”，择优支持
10. 2010年，“天津市市级人才计划入选者”，为天津市特聘教授
2021年以湖北大学图书馆为背景发表的P450综述文章
获选的其中25篇封面文章
